海水铜离子污染对喜盐草生长及抗氧化酶活性的影响
Effect of bivalent copper ion on the growth and antioxidant enzyme activity of Halophila ovalis
李倩茹 1曾思琴 1胡杰飞 1黄俊 1张玉蕾 1曾奇韬 1罗晓霞 1黄翔鹄1
作者信息
- 1. 广东海洋大学水产学院,广东湛江 524000
- 折叠
摘要
为探讨海水中铜离子(Cu2+)污染对喜盐草(Halophila ovalis)生长的影响,通过室内种植喜盐草,然后添加不同质量浓度重金属Cu2+,培养2周后测定了喜盐草生物量、色素含量、可溶性蛋白含量、3种抗氧化酶活性及植物组织内的铜含量.结果显示:不同质量浓度的Cu2+对植株的生物量和相对生长速率影响显著(P＜0.05),对色素含量的影响不显著(P＞0.05);可溶性蛋白含量随着Cu2+质量浓度的增加而增加;超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性随着Cu2+质量浓度的增加而降低;喜盐草地上部分对重金属铜富集量高于地下部分,且随着海水中Cu2+含量的增加富集量增加.研究表明,当海水重金属Cu2+质量浓度超过0.1 mg/L时,喜盐草生长速率、抗氧化酶活性显著降低,可导致海草床退化;重金属铜主要富集于地上部分,可导致喜盐草快速落叶,影响海草床的生态功能.
Abstract
To determine the effect of seawater bivalent copper ion(Cu2+)at different concentrations on the growth of Halophila ovalis,seagrass H.ovalis was planted in door and in seawater containing dif-ferent amounts of Cu2+.After 2 weeks culture,the biomass,pigment content,soluble protein con-tent,three antioxidant enzyme activities of H.ovalis and copper content in its tissues were measured.The results showed that the increase of Cu2+content significantly affected the biomass and relative growth rate(RGR)(P＜0.05).The content of Cu2+had no significant effect on the pigment content(P＞0.05).The soluble protein content increased with the increase of Cu2+content.The activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)decreased with the increase of Cu2+content.The amount of copper in above ground tissues was higher than that in underground parts.Moreover,with the increase of Cu2+content in seawater,more copper was enriched in plant tis-sues.We found also that the growth rate and the antioxidant enzyme activities of H.ovalis were de-creased significantly when the Cu2+was high than 0.1 mg/L in seawater.The high concentrations of Cu2+caused the degradation of seagrass bed.Copper was enriched in the leaves of H.ovalis,leading to the rapid defoliation of H.ovalis and affecting the ecological function of seagrass bed.
关键词
喜盐草/铜/生长/抗氧化酶/富集Key words
Halophila ovalis/copper/growth/antioxidant enzyme/enrichment引用本文复制引用
基金项目
广东省对外科技合作项目(2013B051000081)
广东省大学生创新创业训练计划(CXXL2018051)
广东省自然科学基金(2018A030313212)
出版年
2024
NETL
NSTL
万方数据