Infectious hypodermal and hematopoietic necrosis virus(IHHNV)is one of the main viruses affecting shrimp culture.It is generally believed that IHHNV mainly infects organs originating from ectoderm and mesoderm,and does not infect endoderm organs,such as hepatopancreas.In this study,Penaeus vannamei was artificially infected by feeding challenge,and PCR method was used to analyze the infection of IHHNV to different organs such as gill,hepatopancreas,pleopods and muscle.The re-sults showed that the hepatopancreas of P.vannamei was more susceptible to IHHNV,and the posi-tive infection rate was 86.7％.The virus load of IHHNV was 9.8copies/pL DNA.The infection rate of gill was 46.7％.The viral load of gill IHHNV was 3.0copies/pL DNA.Muscles and pleopods had a similar sensitivity to IHHNV,and the infection rate was 13.3％.The viral load of them was 0.8copies/pL and 0.3copies/p.L DNA,respectively.Our findings showed that the hepatopancreas can also be infected with IHHNV,and the degree of IHHNV infection was higher compared with other or-gans.These results provided a new perspective for the study of the mechanism of IHHNV infection.Due to only the small amount of tissue samples were taken,the histopathological examination of the different organs each shrimp was not carried out.Therefore,the study of IHHNV on the different or-gans of P.vannamei,especially the organs from the endoderm,appreciates further researches in com-bination with histopathology and other molecular biological tools.
infectious hypodermal and hematopoietic necrosis(IHHNV)penaeus vannameipleopodmusclegillhepatopancreas
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NSTL
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