IHHNV对凡纳滨对虾成虾不同器官感染的研究
Studies on infectious hypodermal and hematopoietic necrosis virus(IHHNV)infection in different organs of adult Penaeus vannamei
杨宁 1王静静 1徐家惠 1于吉悦 1李婷 1闫冬春 1常林瑞1
作者信息
- 1. 鲁东大学农学院,山东烟台 264025
- 折叠
摘要
传染性皮下及造血组织坏死病毒(IHHNV)是影响对虾养殖的主要病毒之一.普遍认为IHH-NV主要感染起源于外胚层和中胚层的器官,一般不感染内胚层器官,如肝胰腺.本研究通过室内投喂攻毒的方式对凡纳滨对虾(Penaeus vannamei)进行人工感染,利用PCR方法分析IHHNV对凡纳滨对虾鳃、肝胰腺、游泳足和肌肉不同器官的感染情况.攻毒10 d后结果显示,凡纳滨对虾的肝胰腺在投喂攻毒实验中更易感染IHHNV,其阳性感染率为86.7％,IHHNV病毒载量为9.8 copies/μL DNA.鳃的感染率为46.7％,IHHNV病毒载量为3.0 copies/μL DNA.肌肉和游泳足具有相似的感染性,其感染率都为13.3％,IHHNV病毒载量分别为0.8 copies/μL DNA和0.3 copies/μL DNA.该研究表明来源于内胚层器官肝胰腺也可以感染IHHNV,且与其他器官相比IHHNV感染程度较高,此结果为IH-HNV 感染机制的研究提供了新视点.
Abstract
Infectious hypodermal and hematopoietic necrosis virus(IHHNV)is one of the main viruses affecting shrimp culture.It is generally believed that IHHNV mainly infects organs originating from ectoderm and mesoderm,and does not infect endoderm organs,such as hepatopancreas.In this study,Penaeus vannamei was artificially infected by feeding challenge,and PCR method was used to analyze the infection of IHHNV to different organs such as gill,hepatopancreas,pleopods and muscle.The re-sults showed that the hepatopancreas of P.vannamei was more susceptible to IHHNV,and the posi-tive infection rate was 86.7％.The virus load of IHHNV was 9.8copies/pL DNA.The infection rate of gill was 46.7％.The viral load of gill IHHNV was 3.0copies/pL DNA.Muscles and pleopods had a similar sensitivity to IHHNV,and the infection rate was 13.3％.The viral load of them was 0.8copies/pL and 0.3copies/p.L DNA,respectively.Our findings showed that the hepatopancreas can also be infected with IHHNV,and the degree of IHHNV infection was higher compared with other or-gans.These results provided a new perspective for the study of the mechanism of IHHNV infection.Due to only the small amount of tissue samples were taken,the histopathological examination of the different organs each shrimp was not carried out.Therefore,the study of IHHNV on the different or-gans of P.vannamei,especially the organs from the endoderm,appreciates further researches in com-bination with histopathology and other molecular biological tools.
关键词
传染性皮下及造血组织坏死病毒(IHHNV)/凡纳滨对虾/游泳足/肌肉/鳃/肝胰腺Key words
infectious hypodermal and hematopoietic necrosis(IHHNV)/penaeus vannamei/pleopod/muscle/gill/hepatopancreas引用本文复制引用
出版年
2024
NETL
NSTL
万方数据