Mitochondrial genome characteristics and phylogenetic information of family Engraulidae ( Clupeiformes: Clupeoidei) fishes
In order to understand the mitochondrial genome characteristics and phylogenetic information of family Engraulidae fishes, benefiting the study of evolutionary genetics and selection of molecular markers, all known 10 mitochondrial genomes of Engraulidae fishes were analyzed. The results were as follows: 1 ) Lengths of mitochondrial genomes were from 16 660bp to 17 069bp. Mitochondrial genome composition and gene order was consistent with other teleost fishes; 2) After alignment, 15 704 base pairs of consensus sequences wereobtained,in which 5 570 were variable sites, accounting for 35. 5% of total sites. Among all genes, the ND6 had the highest sequence divergence (47.5% ) and kimura-2-Parameter genetic distance (0.276) , whereas the concatenated tRNA had the lowest sequence divergence (18.7% ) and K -2 -P distance (0.072) ; 3) Protein coding genes were mainly acted by purifying selection which was detected by Z test and Tajima' s D test. Most of the genes, except ND6, were influenced by purifying selection (Ka/Ks <1) ;4) ND4, ND2 and Cytb genes were suitable molecular markers for phylogenetic study of Engraulidae fishes.