Effects of pH on calcification, CA and SOD activities in Cyclina sinensis
pH is an important indicator of water environment and aquatic animals show a series of physiological responses to pH changing.The Venus clam (Cyclina sinensis) is an intertidal clam that is naturally distributed along the coastal muddy sands of China,Korea,Japan and Southeast Asia.C.sinensis farms have been very popular in China since 1990s.At the C.sinensis farm,water pH fluctuate wildly.In order to understand the physiological responses of C.sinensis to various pH changes,the experiment was set up with 5 pH gradients:7.4,7.8,8.2,8.6,9.0 for 7 days.CAT and SOD activities were detected at 0 h,3 h,12 h,24 h,48h,72 h,120 h,168 h after pH treatment.The calcification rate was evaluated at the end of the experiment.Data showed that there was no significant effect in pH 7.8 group compared with the control group (P ＞ 0.05),but in the other groups the calcification rate were significantly depressed (P ＜ 0.05);Significant differences in SOD and CAT activities were only found in pH 7.4 and 9.0 groups (P ＜ 0.05).The enzyme activities raised after pH challenge and then recovered to the initial level in both pH 7.4 and 9.0 groups.The results indicated the C.sinensis could tolerate a wide range of pH,but pH fluctuation still brought significant variances during the growth of C.sinensis.
NETL
NSTL
万方数据
维普
