Effects of feeding level and frequency on growth,serum biochemical indexes,liver and intestinal tissue digestive enzymes of juvenile Selenotoca multifasciata
The is to study the effects of feeding frequency and feeding level on serum physiological and biochemical indexes,digestive enzymes and liver protein metabolism-related enzymes of Selenotoca multifasciata.The feeding frequency experiment used apparent satiety feeding,which set 4 feedings frequencies of 1,2,3,4 times per day,named F1,F2,F3,F4;and four feeding levels were set up:satiety feeding,4％,6％,8％of body weight,xas G1,G2,G3,G4.Juvenile S.multifasciata were selected,and three replicates of each group were used in parallel,with 80 fish in each replicate for 60 d.Results showed:1)Serum biochemical indicator:total protein(TP)and albumin(ALB)contents were the highest in F2,while the remaining groups did not differ significantly(P＞0.05).The content of high-density lipoprotein(HDL)and blood glucose(GLU)were significantly higher at high feeding frequency(P＜0.05).Total protein(TP)and albumin(ALB)levels were significantly higher in G1(P＜0.05).Blood glucose(GLU)content was significantly lower in G1.Triglyceride(TG)content was significantly higher in G2(P＜0.05).The content of low-density lipoprotein(LDL)was significantly lower in G1(P＜0.05).The activity of aspartate transaminase(AST)and alanine aminotransferase(ALT)were significantly(P＜0.05)higher in G1.Alkaline phosphatase(AKP)significantly(P＜0.05)elevated in G2.2)Digestive enzymes:hepatic pepsin activity was significantly higher in F3 and F1 than in F2 and F4(P＜0.05),hepatic trypsin and amylase activities were significantly higher in F3(P＜0.05),and hepatic lipase activity was significantly lower in F4(P＜0.05).Intestinal pepsin activity was significantly lower in F1 group than in F4(P＜0.05),and the difference was not significant in the remaining groups(P＞0.05).Intestinal lipase activity was significantly higher in F4 group,and the difference was not significant(P＞0.05)in F1,F2 and F3.Intestinal amylase activity was significantly(P＜0.05)elevated in F3.Hepatic amylase activity was significantly lower in G3 and G4(P＜0.05).Hepatic pepsin,trypsin and lipase activities were not significantly affected by feeding level(P＞0.05).Intestinal pepsin activity was significantly higher in G1 than in G2(P＜0.05),and pepsin activity was not significantly different among groups G1,G3 and G4(P＞0.05).Intestinal lipase activity was the highest in G1 group.Intestinal amylase activity was significantly higher in G2 group(P＜0.05).The hepatic alanine aminotransferase(ALT)activity was significantly higher in F1 group than in F4 group(P＜0.05),and the lowest was found in F4.Feeding frequency had no significant effect on hepatic aspartate transaminase(AST)activity(P＞0.05).Hepatic alanine aminotransferase(ALT)and aspartate transaminase activities(AST)were significantly higher in G1 than in G2,G3 and G4(P＜0.05).4)Growth:final wet weight,weight gain rate and specific growth rate all achieved the highest in F3,and final wet weight,weight gain rate and specific growth rate were significantly higher in G1 and G4(P＜0.05).Under the present experimental conditions,in terms of growth performance,the optimal feeding frequency for juvenile S.multifasciata was 2 times per day,and feeding level was satiety feeding.
Selenotoca multifasciatafeeding strategyserum physiological and biochemical indicesdigestive enzymes
万方数据
维普
