DNA barcoding and molecular phylogeny of Pteroninae species in China sea
Pteroninae fishes,commonly known as lionfish,are diverse species mainly found in the oceanic waters of tropical,subtropical areas throughout the Indo-Western Pacific.Recently,Pteroninae fishes are still confronted with taxonomic problems because of their identical morphological characteristics.To investigate the availability of mitochondrial cytochrome oxidase subunit Ⅰ(CO Ⅰ)sequences as DNA barcodes for Pteroinae species identification,partial CO Ⅰ sequences of 8 Pteroinae speices from China were obtained by PCR amplification and sequencing.Combining with sequences obtained from GenBank,CO Ⅰ sequences of 40 individuals in 14 Pteroinae species were analyzed.The sequence information and genetic distances were calculated using MEGA X,molecular phylogenetic trees were constructed and analyzed basing on the maximum likelihood and neighbour-joining method.The result showed that:In the length of 639 bp consensus sequence,the base composition was A:23.7％,T:29.6％,C:26.8％and G:19.8％.421 sites were conservative(65.9％),218 sites were variable(34.1％)and 191 sites were parsim-informative(29.9％).In genetic distance,except for one each undeterminable sample of Ebosia bleekeri and Pteroidichthys amboinensis,the average intra-species genetic distance values of the rest 12 Pteroinae species was 0.002 4;while the average inter-species genetic distance was 0.166,which was 69.17 times of the former,revealing that CO Ⅰ gene could be used as an effective DNA barcode gene for identifying Pteroinae species.In Pteroinae molecular phylogenetic trees,except for genus Pteroidichthys,which was first separated and located at the base of the tree,other genera in Pteroinae could not form a monophyletic group,especially the species-rich genera Pterois and Dendrochirus,forming more branches in the tree.In the genus Pterois,two independent branches formed,two species:P.volitans and P.miles,tightly clustered together as one branch,indicating their close relationship,but the CO Ⅰ genetic distance values were 0.042,greater than the minimum distance value(0.020)for species identification recommended by Hebert,supporting the recent opinions that they might be independent species.The other three Pterois species P.antennata,P.radiate and P.mombasae clustered as another branch,independent to the cluster(P.volitans+P.miles)and closely related to Dendrochirus zebra and Ebosia species.Such relationships were also found in the recent molecular phylogenetic studies,indicating that they might be two independent groups in Pterois.However,whether the three Pterois species would be seperated from Pterois and classified into genus Pteropterus,more Pteroinae species samples should be collected and analyzed in further studies.
万方数据
维普
