Mechanism of salinity stress on oxidative damage in the liver of Larimichthys crocea
Large yellow croaker Larimichthys crocea is a main commercial marine fish,output is the highest among sea cage-cultured fishes in China.In order to explore the mechanism of salinity stress on oxidative damage of L.crocea,three salinity gradients(12,25,40,respectively recorded as L,C,H)were set up in the experiment.L.crocea was subjected to salinity stress experiment,and samples were taken on the 1st,3rd and 7th days of the experiment.The results showed that the contents of malondialdehyde(MDA)and lipid peroxide(LPO)in the liver of L.crocea increased significantly on the 1st,3rd and 7th days compared with that of the control(P＜0.05).The activities of superoxide dismutase(SOD)were all significantly higher than the control on the 1 st day,then decreased and were all significantly lower than the control on the 7 th day(P＜0.05).The catalase(CAT)of the liver were siginificantly lower than the control on the 1st and 7th days(P＜0.05),and showed no significant difference among groups on the 3rd day(P＜0.05).Lysozyme(LZM)activities generally showed a similar trend with SOD.The alkaline phosphatase(AKP)activities showed a complex changing trend.The gene expression levels of copper zinc superoxide dismutase(Cu/Zn-SOD),manganese superoxide dismutase(Mn-SOD),CAT,c-type LZM,g-type LZM and AKP were positively correlated with corresponding enzyme activities(P＜0.05);NF-E2 related factor 2(Nrf2)and nuclear transcription factor-κ B(NF-κB)gene expression levels were positively correlated with the expression levels of antioxidant enzyme genes and non-specific immune enzyme genes,respectively(P＜0.05).In sum,L.crocea could enhance salinity stress tolerance by improving antioxidant capacity and non-specific immunity.
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