Bioinformatics software was used to screen and analyze the distribution pattern of microsatellite sequences in the genome of Neophocaena asiaeorientalis asiaeorientalis.The results showed that:1)A total of 4 431 492 intact SSR loci were found in the microsatellites of Neophocaena asiaeorientalis asiaeorientalis,with a total length of 83 876 118 bp,accounting for approximately 0.41％of the total sequence.2)The proportion of single base,two-base,three-base,four-base,six-base,and five-base repeat types decreased sequentially,with the proportions of 64.77％,21.48％,5.14％,4.60％,3.60％,and 0.41％,respectively;Neophocaena asiaeorientalis asiaeorientalis genome contained a total of 209 repetitive base elements.Among them,there were 2,4,9,29,67,98 types of single base repeat,two-base repeat,three-base repeat,four-base repeat,five-base repeat,and six-base repeats respectively.3)In the composition of microsatellite dominant repeat bases,single base repeat(A/T)accounted for the largest proportion of total SSR(62.67％),96.76％of single base repeat types,and was in an absolute dominant position;the second was the double base repeat unit(AC/TG),which accounted for 15.33％of total SSR;the proportion of six-base repeat units(AATCCC/TTAGGG)in the total SSR was 4.49％;the proportions of four-base repeat units(AAAT/TTTA),three-base repeat units(AAT/TTA),and five-base repeat units(AAAAC/TTTTG)in the total SSR were 1.58％,1.04％,and 0.19％,respectively,indicating that the microsatellite characteristics of Neophocaena asiaeorientalis asiaeorientalis had a preference for AT.4)The number of microsatellites decreased with the increase of base repeats,and there was no significant difference in the length of microsatellite fragments.The fragment length range was 12-50 bp,with an average length of 18.93 bp,belonging to medium to high polymorphism.The results of this study provide certain data support for the subsequent development and screening of high-quality microsatellite markers of Phocaenidae for Neophocaena asiaeorientalis asiaeorientalis genome,thereby contributing to the study of genetic diversity and structure of Neophocaena asiaeorientalis asiaeorientatis population.
维普
万方数据
