Analysis of antioxidant capacity and weighted gene co-expression network of related genes in grouper(Epinephelus lanceolatus♂ × E.fuscoguttatus ♀)under the regulation of Haematococcus pluvialis astaxanthin
In order to study the effects of Haematococcus pluvialis astaxanthin on the growth and antioxidant capacity of E.lanceolatus ♂ × E.fuscoguttatus ♀,three types of feeds containing astaxanthin 0 mg·kg-1(control group),48.56 mg·kg-1(low-dose group)and 189.56 mg·kg-1(high-dose group)were prepared.The growth performance and serum biochemical parameters of the grouper were examined after a 60-day feeding experiment.The antioxidant related co-expression gene modules were idenified by combination of differentially expression genes(DEGs)and WGCNA.The hub genes were identified according to the connectivity of genes in the modules.The results showed that the final body weight(FBW),weight gain rate(WGR),specific growth rate(SGR),and the activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-PX)were significantly higher in experimented groups than those in the control group(P＜0.05),while the content of malondialdehyde(MDA)was significantly lower in experimented groups(P＜0.05).A total of 3 089 and 3 012 DEGs were identified in the low and high dose groups,respectively.These DEGs were mainly enriched in Jak-STAT,chemokine,and AMPK signaling pathway.Co-expression analysis identified 17 modules,in which four(black,turquoise,magenta,and light green)were significantly associated with antioxidants.KEGG functional enrichment analyses were performed based on these four specific modules,which could be enriched in Jak-STAT signaling pathway and autophagy pathway.The top 20 hub genes were selected by calculating the gene connectivity in the corresponding networks,and 14 hub genes were finally screened by functional annotation.Transcription factors such as zf-C2H2、zf-BED and zf-MIZ were also included in the co-expression network.In sum,Haematococcus pluvialis astaxanthin can improve the growth performance and antioxidant capacity of E.lanceolatus ♂ × E.fuscoguttatus ♀.The hub genes may be crucial in the control of antioxidants,which provides references for the development of more effective grouper feed.
万方数据
维普
