Study on response of Exopalaemon carinicauda to low temperature stress based on sRNA
Studying the effects of low temperature stress on the expression of microRNA(miRNA)and its corresponding target genes in Exopalemon carinicauda,and screening for miRNAs that may be associated with low temperature regulation will help reveal the role of miRNAs in the mechanism of low temperature tolerance.E.carinicauda with a body weight of(1.27±0.15)g was subjected to low temperature(0℃)treatment for 4 h,the control group was maintained at 25 ℃,and a 20.45M bp filtered sequence was obtained through sRNA sequencing.Through comparison,55 conserved miRNAs and 9 non conserved miRNAs were obtained.Quantitative analysis of miRNA expression abundances in E.carinicauda under low temperature stress revealed 11 significantly up-regulated miRNAs,of which dme-miR-100-3p was the most up-regulated miRNA after low temperature stress,and its corresponding target genes were mainly potassium channel and serine protease inhibitor.The miRNA with the second highest up-regulation after low temperature stress was dme-miR-2491-5p,which corresponded to target genes such as acetyllactate synthase and sodium/glucose co transporter protein.The miRNA with the third highest up-regulation after low temperature stress was rno miR-675-3p,and the corresponding target genes mainly included amino acid transporters and alkaline phosphatase.In addition,miRNA-hsa-miR-671-3p was significantly up-regulated after low temperature stress,and its corresponding target genes were mainly lactate dehydrogenase and glucosidase.In summary,E.carinicauda responded positively to temperature changes after low temperature stress by activating signaling pathways such as carbohydrate and protein metabolism,mobilizing various glucose and amino acid metabolism in the body to provide energy to resist low temperature stress and maintain body homeostasis.599 target genes showed significant differential expression.According to GO classification and enrichment analysis,it was found that the differentially expressed miRNAs were significantly regulated by target genes such as stimulus response,biological regulation,adhesion,catalytic activity and metabolic process.COG classification found that differentially expressed miRNAs significantly regulated target genes such as post translational modification,protein turnover and chaperone protein,carbohydrate transport and metabolism,and amino acid transport and metabolism.KEGG enrichment analysis found that the target genes dihydrothiooctyl dehydrogenase and lactate dehydrogenase were enriched in glycolysis/gluconeogenesis,pyruvate metabolism,amino acid metabolism and degradation,and the target gene E1A binding protein was enriched in transforming growth factor-β signaling pathway,Wnt signaling pathway and JAK-STAT signaling pathway,while the target gene calcium/calmodulin dependent protein kinase was enriched in Wnt signaling pathway,calcium signaling pathway and ErbB signaling pathway.It can be seen that differentially expressed miRNAs and related target genes play an important role in the regulation of transmembrane signal transduction,protein metabolism,and carbohydrate metabolism in Exopalaemon carinicauda under low temperature conditions.Therefore,it is speculated that low temperature can adapt to stress by upregulating cell glycolysis and promoting the decomposition of substrates such as proteins and carbohydrates through tricarboxylic acid cycle pathway by miRNAs in E.carinicauda.This study lays the foundation for further exploring the differential expression and function of miRNA under low temperature stress in E.carinicauda.
low temperature stresssRNAmiRNAExopalaemon carinicauda
万方数据
维普
