Effects of cadmium on neural and endocrine systems and oxidative stress of Hexagrammos otakii
Cadmium(Cd)is a heavy metal element and has strong accumulation for organisms.The accumulation of cadmium in vivo can cause serious toxic effects on various physiological functions.The toxicity of cadmium is also related to the production of abundant reactive oxygen species(ROS)in cells,causing oxidative damage.Hexagrammos otakii is a common reef fish in the Yellow Sea and Bohai Sea,with high economic and ecological value.It is often used as a target organism in the investigation and evaluation of cadmium pollution in sea areas.The fish were cultured in seawater at three Cd2+concentrations(0.2,2.5,10.0 μg·L-1)for 12,24 d,and 0 μg·L-1 Cd2+concerctration was considered as blank control.After the breeding experiment,acetylcholinesterase(AchE)activities in the brain and cortisol contents in the serum were investigated to explore the effects of cadmium exposure on neural and endocrine systems of the fish;meanwhile,superoxide dismutase(SOD)activities,catalase(CAT)activities,glutathione s transferase(GST)activities and malondialdehyde(MDA)contents in the serum were investigated to explore the effects of cadmium exposure on oxidative stress response of the fish.The results showed that:1)Cd exposure showed obvious toxicity on the neural and endocrine systems of H.otakii,when exposed for 12 h and 24 h,the AchE activities and Cor content in all treatment groups were significantly lower than that of the control(P＜0.05).2)Cd exposure could increase the SOD,CAT,GST activities and MDA content of H.otakii,but the effect time was different;when exposed for 12 h,GST activities of all treatment groups were significantly higher than that of the control(P＜0.05),then GST activities of treatment groups decreased gradually,at 24 h,GST activities of 0.2,10.0 μg·L-1 groups were significantly lower than that of the control(P＜0.05);when exposed for 24 h,SOD,CAT activities and MDA content of all treatment groups were significantly higher than that of the control(P＜0.05).The results also indicated that acute Cd exposure could trigger oxidation stress response in H.otakii.The antioxidant function of GST enzymes decreased after Cd exposure for 24 h,resulting in accumulation of MDA.The toxicity of Cd exposre showed no obvious concentration effect.The results can provide support for the Cd risk analysis of H.otakii and references for the marine environment monitoring evaluation and fishery resource protection.
万方数据
维普
