为了将绵羊体外胚胎发育技术应用于生产,采用活体采卵-体外受精的方法,对准备淘汰的杜泊羊进行外源激素处理,并对可能影响体外胚胎发育及移植后受胎效率如FSH剂量、公羊个体、获取卵母细胞方式等因素进行研究,结果发现:(1)每只供体注射FSH 240 IU或420 IU剂量对获得卵母细胞数、胚胎数及体外受精卵裂率没有明显影响(P＞0.05);(2)不同公羊个体对卵母细胞体外受精效率有显著影响,但胚胎移植后受胎率没有明显影响(P＞0.05);(3)活体采卵与屠宰场采卵方式相比,对卵母细胞的回收没有影响(12.24 vs 13.84,P＞0.05),但活体采集卵母细胞受精后的平均胚胎数显著低于后者(7.50 vs 11.17,P＜0.05),卵裂率极显著低于屠宰场采卵方式(60.92％ vs 81.75％,P＜0.01);(4)上述几组试验对受胎率都没有影响(P＞0.05).由此可见,供体使用240 IU的FSH,选择受精效率好的公羊,能显著提高杜泊羊体外胚胎生产效率;离体采集卵母细胞体外发育效率高于活体采卵.
Factors affecting efficiency of in vitro embryo production in Dorper sheep
In order to apply the technology of in vitro lamb embryo development to production,we studied the possible effects of factors such as dose of FSH,individual rams,oocyte obtain methods on in vitro embryo development and the conception efficiency after transplantation.The results showed that injection dose of FSH 240 IU or 420 IU per donor had no effect on the number of obtained oocytes,the number of embryos,and in vitro fertilization and cleavage rate (P＞0.05).Ram had significant effects on in vitro oocyte fertilization efficiency (P ＜ 0.05),but did not affect the pregnancy rate of embryo transplantation (P＞0.05).When compare oocyte harvesting method of ovum pick up (OPU) with slaughterhouse,there was no effect on the recovery of oocyte (12.24 vs.13.84,P＞0.05).However,the average number of embryos obtained by OPU was significantly lower than that obtained by slaughterhouse (7.50 vs 11.17,P＜0.05),and the cleavage rate was extremely significantly lower than the latter (60.92％ vs 81.75％,P＜0.01).All of the above tests had no effect on the pregnancy rate (P＞0.05).Using 240 IU donor FSH and selecting the fertilization efficiency ram,can significantly improve the in vitro embryo production.The in vitro developmental ability of the oocyte harvested by the slaughterhouse was higher than that by OPU.
NETL
NSTL
万方数据
维普
