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2株节旋藻的分类鉴定和磷脂脂肪酸特征分析

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利用荧光显微镜观察节旋藻CB001和CB002的菌丝形态,同时对其16S rDNA序列以及在液体和固体培养条件下的磷脂脂肪酸特征进行系统分析。显微成像观察表明,CB001和CB002均属于钝顶节旋藻(Arthrospira platensis),具有运动能力;磷脂脂肪酸特征分析结果显示,磷脂脂肪酸16∶0、16∶1 w7c、18∶1w9c、18∶2 w6c、14∶0 anteiso和16∶1 w7c alcohol可能是节旋藻的代表性脂肪酸;18∶1 w5c和16∶3 w6c分别为CB001和CB002的特有磷脂脂肪酸组分;同时,不同培养方式对CB001和CB002磷脂脂肪酸组成模式具有极其显著的影响。
Characterization of phospholipid fatty acid of two Arthrospira strains
The morphology,16S rDNA and phospholipid fatty acid composition of two isolates including CB001 and CB002 cultured in liquid medium and on the solid plate was studied.The result of morphology and phylogeny analysis confirmed that both CB001 and CB002 were belonged to Arthrospira platensis.The result of fatty acid composition analysis showed that 16 ∶ 00,16 ∶ 1 w7c,18 ∶ 1 w9c,18 ∶ 2 w6c,14 ∶ 0 anteiso and 16 ∶ 1 w7c alcohol were the main PLFAs in the genus Arthrospira.18 ∶ 1 w5c and 16 ∶ 3 w6c were the characteristic PLFA of strain CB001 and CB002,respectively.The PLFA patterns of both CB001 and CB002 were affected significantly by culture conditions.Further application of PLFA technique could be used to identify and classify Arthrospira,or estimate biomass or purity in large-scale bacterial proliferation.

cyanobacteriaphylogenyphospholipid fatty acidArthrospira

杜改利、汪方奎、胡甜、王莉、陈雯莉

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华中农业大学农业微生物学国家重点实验室,武汉430070

蓝细菌 系统发育 磷脂脂肪酸分析 节旋藻

国家自然科学基金中央高校基本科研业务费专项

315700482014PY003

2016

华中农业大学学报
华中农业大学

华中农业大学学报

CSTPCDCSCD北大核心
影响因子:1.09
ISSN:1000-2421
年,卷(期):2016.35(6)
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