不同生育期番茄植株根际土壤微生物群落结构特征
Structural characteristics of soil microbial community in rhizospheres of tomatoes during different growth periods
魏宇飞 1覃仁柳 1丁点草 1黎永斌 1谢源源 1屈达才 1赵天义 2杨尚东1
作者信息
- 1. 广西大学农学院/植物科学国家实验教学示范中心,南宁 530004
- 2. 广西钦州市农业技术推广中心,钦州 535099
- 折叠
摘要
为构建番茄生态优质高产栽培技术体系及挖掘有益的微生物资源,比较了不同生育期番茄植株根际土壤肥力以及微生物群落结构与功能特征.基于传统与现代高通量测序技术分析番茄幼苗期、花芽分化期、结果期根际土壤肥力与微生物(细菌、真菌)群落结构与功能特征.结果显示,番茄结果期根际土壤中具有更为丰富的碳、氮、磷源;另一方面,不同生育期番茄根际土壤微生物群落结构和功能发生显著变化.其中,norank_f_norank_o_norank_c_KD4-96,norank_f_A4b和Bryobacter属细菌是幼苗期番茄植株根际土壤中特有的优势细菌属;TM7a和Saccharomonospora属细菌是花芽分化期番茄植株根际土壤中特有的优势细菌属;Gem-matimonas属细菌是结果期番茄植株根际土壤中特有的优势细菌属;此外,鲍尔壶菌属(Powellomyces)真菌是幼苗期番茄植株根际土壤中特有的优势真菌属;Apiotrichum和unclassified_f_Chytridiaceae真菌是花芽分化期番茄植株根际土壤中特有的优势真菌属;链格孢属(Alternaria)真菌是结果期番茄植株根际土壤中特有的优势真菌属.结果表明:不同生育期番茄植株根际土壤中形成了特异的根际土壤微生物群落,幼苗期根际土壤中主要富集了可以产生生长激素的微生物类群;花芽分化期根际土壤中主要富集了具有抗逆功能的微生物类群;结果期根际土壤中主要富集了具有促进养分循环功能的微生物类群.
Abstract
The soil fertility and structural and functional characteristics of microbial community in rhi-zospheres of tomatoes during different growth periods were compared to construct an eco-cultivation system for tomato production with high yield and high quality and utilize the resources of beneficial microbes.The soil fertility and structural and functional characteristics of microbial(bacterial,fungal)community in rhizo-spheres of tomato at the stage of seedling,flower and bud differentiation,and fruiting were analyzed based on conventional and modern high-throughput sequencing techniques.The results showed that there were more abundant sources of carbon,nitrogen,and phosphorus in the rhizospheres of of tomatoes at the stage of fruiting in tomato.The structure and function of soil microbial community in rhizospheres of tomatoes had undergone significant changes during different growth periods.Among them,norank_f_norank_o_norank_c_KD4-96,norank_f_A4b and Bryobacter were the unique dominant bacterial genus in the rhizo-spheres of tomatoes at the stage of seedling.TM7a and Saccharomonospora were the unique dominant bac-terial genus in rhizospheres of tomatoes at the stage of flower and bud differentiation.Only Gemmatimonas was the unique dominant bacterial genus in rhizospheres of tomatoes at the stage of fruiting.Moreover,Powellomyces was the unique dominant fungal genus in rhizospheres of tomatoes at the stage of seedling,Apiotrichum and unclassified_f_Chytridiaceae were the unique dominant fungal genera in rhizospheres of to-matoes at the stage of flower and bud differentiation,and Alternaria was the unique dominant fungal genus in rhizospheres of tomatoes at the stage of fruiting.It is indicated that specific microbial communities were formed in rhizospheres of tomatoes at different stages of growth.Microorganisms enriched in rhizospheres of tomatoes at the stage of seedling mainly produce hormones for plant growth.The enrichments of soil mi-croorganisms in rhizospheres of tomatoes at the stage of flower and bud differentiation primarily improve re-sistance for plants.Microorganisms enriched in rhizospheres of tomatoes at the stage of fruiting chiefly pro-mote the nutrient cycling in soil.
关键词
番茄/生育期/根际/微生物群落/高通量测序Key words
tomato(Solanum lycopersicum L.)/growth period/rhizosphere/microbial community/high throughput sequencing引用本文复制引用
基金项目
国家自然科学基金(31860547)
广西壮族自治区重点研发计划(桂科AB23026076)
出版年
2024
NETL
NSTL
维普
万方数据