为明确番茄接种根结线虫后基因种类和表达量在转录水平的变化规律，利用RNA-seq对未接种及接种南方根结线虫2龄幼虫6、12、24和48 h的番茄进行转录组测序，探究番茄响应南方根结线虫侵染相关的关键转录因子，并采用qRT-PCR方法对测序结果进行验证。结果显示，接种南方根结线虫后6、12、24和48 h分别有350、390、580、1 154个基因差异表达，其中差异表达转录因子分别为11、11、19、50个。这些转录因子属于15个家族，其中数量最多的为MYB家族和bHLH家族(均为20个)，其次是ERF家族19个、WRKY家族15个、bZIP家族9个。南方根结线虫侵染过程差异表达最明显的主要为ERF、WRKY、MYB和bHLH家族转录因子，其中Solyc03g005520、Solyc02g094270和Solyc09g066350显著上调，接种后48 h log2FC分别为 9。16、6。49和6。33;Solyc02g079280、Solyc12g100140 和Solyc04g072460显著下调，接种后48 h log2FC分别为-2。60、-1。72和-1。70。qRT-PCR验证结果显示，6个随机选取基因的表达趋势与测序结果基本一致。以上结果表明，ERF、WRKY、MYB和bHLH家族转录因子可能参与番茄与南方根结线虫互作，在番茄响应南方根结线虫侵染反应中发挥着重要的调控作用。
Preliminary analysis of transcription factors associated with tomato response to infection of Meloidogyne incognita
In order to explore the gene expression pattern at transcriptional level of tomato after inocu-lated with Meloidogyne incognita,transcriptome sequencing was performed on tomato root at 6 h,12 h,24 h and 48 h after inoculated with 2nd instar larvae of root-knot nematode.The key transcription factor re-lated to the tomato response to the infection of M.incognita were analyzed and the sequencing results were verified by quantitative real-time PCR.The results showed that 350,390,580,1 154 genes were differen-tially expressed at 6 h,12 h,24 h and 48 h after inoculation,and 11,11,19 and 50 transcription factors were differentially expressed,respectively.They belonged to 15 transcription factor families,of which MYB family and bHLH family are the most abundant with 20 genes,followed by ERF family with 19 genes,WRKY family with 15 genes,and bZIP family with 9 genes.Further studies showed that the tran-scription factors of ERF,WRKY,MYB and bHLH family were the most differentially expressed.Solyc03g005520,Solyc02g094270 and Solyc09g066350 were significantly up regulated and the log2FC at 48 h after inoculation was 9.16,6.49 and 6.33,respectively.Solyc03g005520,Solyc02g094270 and Solyc09g066350 were significantly down regulated,and log2FC at 48 h after inoculation was-2.60,-1.72 and-1.70,respectively.The results of qRT-PCR analysis showed that the expression trend of six random-ly selected genes was consistent with the sequencing results.The results of this study suggest that the tran-scription factors of ERF,WRKY and bHLH family may be involved in the interaction between tomato and M.incognita,and play an important regulatory role in tomato response to the infection of M.incognita.
NETL
NSTL
万方数据
维普
