核酸酶基因LoENDONUCLEASE 1在东方百合花朵衰老过程中的功能分析
Function analysis of nuclease gene LoENDONUCLEASE 1 during flower senescence in Lilium oriental hybrid
李蕊蕊 1刘玉杰 1许鑫彤 1刘熠 1何颖姣 1王彩云 1罗靖1
作者信息
- 1. 果蔬园艺作物种质创新与利用全国重点实验室/华中农业大学园艺林学学院,武汉 430070
- 折叠
摘要
为明确百合(Lilium spp.)花朵衰老发生的分子机制,在东方百合'西伯利亚'(Lilium oriental hybrid'Siberia')花被片中克隆了一个衰老相关基因LoENDONUCLEASE 1(SAG10),利用qRT-PCR进行基因表达分析,并通过农杆菌介导的拟南芥稳定表达系统和百合花被片瞬时表达体系验证LoENDONUCLEASE 1基因功能.结果显示,LoENDONUCLEASE 1基因开放阅读框(ORF)为921 bp,编码306个氨基酸;LoENDONU-CLEASE 1在百合花朵和叶片的衰老过程中特异表达,且受到脱落酸和水杨酸的诱导;拟南芥过表达LoENDO-NUCLEASE 1株系中叶片叶绿素含量显著低于对照株系,百合花被片中瞬时过表达LoENDONUCLEASE 1基因加速了百合花被片的衰老,且过表达花被片中丙二醛和离子渗透率含量显著高于对照.结果表明,LoEN-DONUCLEASE 1具有促进花朵和叶片衰老的功能.
Abstract
A senescence-related gene LoENDONUCLEASE 1(SAG10)was cloned from the tepals of Lilium oriental hybrid'Siberia'to study the molecular mechanism underlying the senescence of flower in lily(Lilium spp.).The expression of SAG10 was analyzed with qRT-PCR,and the function of LoEN-DONUCLEASE 1 gene was identified with Agrobacterium-mediated stable overexpression system of Ara-bidopsis thaliana and transient overexpression system of lily tepals.The results showed that the open read-ing frame(ORF)of LoENDONUCLEASE 1 gene was 921 bp,encoding 306 amino acids.LoENDONU-CLEASE 1 was uniquely expressed in the senescence of flowers and leaves in lily,and was induced by ab-scisic acid and salicylic acid.The content of chlorophyll in leaves of Arabidopsis strain with overexpressing LoENDONUCLEASE 1 was significantly lower than that in the control.LoENDONUCLEASE 1 gene was transiently overexpressed in lily tepals,accelerating the senescence of tepals in lily.The content of malondialdehyde(MDA)and ion leakage rate in the overexpressed tepals were significantly higher than that in the control.It is indicated that LoENDONUCLEASE 1 can promote the senescence of flowers and leaves in lily.
关键词
百合/鲜切花/花朵衰老/衰老相关基因/核酸酶/过表达Key words
lily/cut flowers/flower senescence/senescence-associated genes/nuclease/overexpres-sion引用本文复制引用
基金项目
国家重点研发计划(2018YFD1000407)
中央高校基本科研业务费专项(2662015BQ008)
国家自然科学基金(31401911)
国家自然科学基金(32172611)
出版年
2024
NETL
NSTL
万方数据