Effects of adding Clostridium butyricum to soybean meal replacing fish meal on growth performance,body composition and antioxidant capacity of largemouth bass(Micropterus salmoides)
In order to develop a high-quality formula feed for largemouth bass(Micropterus salmoi-des),this study replaced fish meal with soybean meal and added probiotics to investigate the growth and antioxidant tolerance of fish.Juvenile largemouth bass with initial body weight(50.78±1.15)g were used as the research object.The fish meal content of the control group was set to 30%,and on the basis of the control group,the fish meal content of the treatment group was replaced with 25%,50%and 75%soy-bean meal,respectively,which were recorded as D25,D50 and D75 treatment groups.In addition,0.05%Clostridium butyricum was added to 3 treatment groups D25+C,D50+C and D75+C.After 8 weeks of experiment,the growth performance,feed utilization,body composition and antioxidant perfor-mance of largemouth bass were analyzed.The results showed that when 25%fish meal was replaced by soybean meal,there were no significant differences in growth performance,body composition and antioxi-dant capacity of largemouth bass(P>0.05).But weight gain rate(WGR)and specific growth rate(SGR)decreased significantly(P<0.05),and feed conversion ratio(FCR)increased significantly when the replacement level of soybean meal reached 50%(P<0.05).The oxygen species(ROS),total antioxi-dant capacity(T-AOC)and superoxide dismutase(SOD)in liver and serum were also significantly changed(P<0.05).However,there were no significant differences in WGR,SGR,ROS,T-AOC and SOD between the group supplemented with C.butyricum on the basis of replacing 50%fish meal and the control group.When the replacement level reached 75%,the growth performance and antioxidant perfor-mance of fish were improved by adding C.butyricum,but still significantly lower than that of the control group.To sum up,the addition of 0.05%C.butyricum could improve the negative effects of 50%substitu-tion of soybean meal for fish meal on the growth performance and antioxidant capacity of largemouth bass.
NETL
NSTL
万方数据
维普
