Purification and activity analysis of Toxoplasma gondii MIC1 protein expressed in baculovirus system
The purpose of this study was to construct a recombinant baculovirus strain expressing solu-ble Toxoplasma gondii MIC1 protein and identify the biological activity of the recombinant protein.The CDS sequence of mic1 gene was amplified by PCR,ligated into the pFastBac 1 vector,and then trans-formed into DH10Bac cell.To obtain recombinant baculovirus,recombinant Bacmid was extracted and transfected into Sf9 cells.The transfected Sf9 cells exhibited typical lesion on the 3rd day after transfection.The results of indirect immunofluorescence assay and Western blot showed that the MIC1 recombinant pro-tein was successfully expressed in transfected Sf9 cells.The purified MIC1 recombinant protein not only possessed the ability to bind to sialyllactose,but also could stimulate Balb/c mice to produce a high level of specific antibodies(>1∶25 600).These results showed that the biologically active MIC1 recombinant pro-tein was successfully obtained through the baculovirus expression system.
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NSTL
万方数据
维普
