玉米籽粒发育突变体emp35的表型分析与基因定位
Phenotype identification and gene mapping of maize kernel development mutant emp35
刘津 1汤艳芳 2杜何为 3张祖新4
作者信息
- 1. 长江大学生命科学学院,荆州 434023;华中农业大学作物遗传改良全国重点实验室,武汉 430070
- 2. 湖北中医药大学检验学院,武汉 430065
- 3. 长江大学生命科学学院,荆州 434023
- 4. 华中农业大学作物遗传改良全国重点实验室,武汉 430070
- 折叠
摘要
为解析玉米籽粒形成的遗传基础,探究Emp35基因在玉米籽粒发育中的作用,对籽粒缺陷突变体empty pericarp35(emp35)进行表型鉴定、胚乳细胞显微观察、胚乳贮藏物质含量测定及图位克隆.结果显示:突变体籽粒发育缓慢,明显小于同期发育的正常籽粒,成熟籽粒干瘪呈空皮状;胚乳细胞显微观察发现emp35的胚和胚乳发育严重滞后,胚乳细胞中线粒体结构异常;淀粉和蛋白质积累减少;F2代分离果穗上正常籽粒与发育缺陷籽粒呈3∶1分离,表明籽粒缺陷表型由单个隐性核基因突变所致.采用集团分离分析法(bulked segregant analysis,BSA)将Emp35定位于第8染色体 127.90～163.36 Mb区间,在该区间内开发了4个InDel标记,连锁作图将Emp35精细定位于139 571 117～146 176 858区间.
Abstract
The phenotype identification,microscopic observation of endosperm cells,determining the content of storage substance in endosperm,and map-based cloning of a defective maize kernel mutant named as empty pericarp35(emp35)were conducted to study its physiological function in the development of maize kernel and map the Emp35 gene.The results showed that the development of emp35 kernels was slow and significantly smaller than that of the wild-type kernels during the same period of development,re-sulted in the shrunken and empty pericarp kernels.The development of embryo and endosperm in the emp35 was severely delayed,and the mitochondrial structure in endosperm cells was abnormal.The accu-mulation of starch and protein in the emp35 was reduced.The seeds in the F2 population segregated in a ra-tio of 3 wild type to 1 empty pericarp,indicating that the defective kernel phenotype is controlled by a sin-gle recessive nuclear gene.The Emp35 gene was mapped at the interval between 127.90 Mb and 163.36 Mb on the chromosome 8 of maize with a bulked segregant analysis(BSA).Four InDel markers were de-veloped within this interval,and Emp35 gene was finely mapped at the interval between 139 571 117-146 176 858.
关键词
玉米(Zea/mays/L.)/籽粒发育/集团分离分析法/基因定位/表型鉴定Key words
maize(Zea mays L.)/kernel development/bulked segregant analysis(BSA)/gene mapping/phenotype identification引用本文复制引用
基金项目
国家自然科学基金(32072069)
湖北省自然科学基金创新群体项目(2022CFA030)
湖北洪山实验室重大项目(2022hszd029)
出版年
2024
NETL
NSTL
万方数据