Effects of culture conditions and exogenous additives on cadmium content in mycelia and fruiting body of Lentinula edodes
Abastract In order to explore the method of inhibiting cadmium enrichment of L.edodes and develop the technology of preventing and controlling cadmium pollution,in this paper,two methods of mycelium cul-ture in laboratory and bag culture in greenhouse were set up to analyze the effects of culture conditions and ex-ogenous additives on the content of cadmium in mycelia and fruiting body of L.edodes.The results showed that in the range of 10-30℃,with the increase of culture temperature,the cadmium content of mycelium in-creased first and then stabilized.In the range of pH 4-8,with the increase of the pH value of the medium,the cadmium content of mycelium first increased and then decreased.Mg2+ could significantly inhibit the enrich-ment of cadmium in mycelia when 5 mmol/L Ca(NO3)2,KNO3 and Mg(NO3)2 were added to the liquid medi-um containing cadmium.As the fruiting bodies of L.edodes matured,cadmium content decreased gradually and the content of cadmium in different parts showed gill>cap>stipe.When magnesium sulfate,zinc sul-fate,activated carbon and zeolite were added,it was found that 50 mg/kg magnesium sulfate,60 mg/kg zinc nitrate and 2.5%activated carbon could reduce the content of cadmium in fruiting body by 42.8%,46.9%and 50.3%.The above research showed that culture conditions and exogenous additives had certain effects on the cadmium content in L.edodes at mycelium stage and fruiting stage.The enrichment of cadmium in L.edodes could be inhibited by exogenous additives.
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