芥菜小孢子培养及染色体加倍技术体系的优化
Optimization of microspore culture system and chromosome doubling technology in mustard
储玉凡 1陈野 1付文苑 2姚培杰 1张志琪 1兰凯 1余长春 1万正杰1
作者信息
- 1. 华中农业大学园艺林学学院/果蔬园艺作物种质创新与利用全国重点实验室,武汉 430070
- 2. 华中农业大学园艺林学学院/果蔬园艺作物种质创新与利用全国重点实验室,武汉 430070;贵州省农业科学院园艺研究所,贵阳 550006
- 折叠
摘要
为探究不同芥菜基因型和培养条件对小孢子培养效果的影响,建立适合芥菜小孢子培养及染色体加倍的最佳方案,对芥菜8个不同变种的34份自交系进行小孢子培养,比较不同热激时间、活性炭浓度、小孢子密度对出胚率的影响.结果显示,基因型对芥菜小孢子培养成功与否影响较大,34份材料中11份材料成功培养出胚状体;不同材料间出胚效果差异明显,其中,大头菜出胚率最高,可达23.85胚/蕾;芥菜小孢子出胚最佳条件为32℃热激1～2 d,培养基含活性炭3～5 g/L,小孢子密度为1.5×105～2.0×105 个/mL.染色体加倍试验结果显示,1 g/L秋水仙素溶液浸泡茎尖1 h的处理加倍效率高、嵌合体少,可应用于芥菜染色体的加倍.
Abstract
The effects of genotypes and culture conditions on the microspore culture in mustard(Bras-sica juncea)were studied to establish an optimal system of the microspore culture and chromosome dou-bling in mustard.34 inbred lines of 8 different varieties of mustard were used to conduct the microspore cul-ture.The effects of heat shock times,concentrations of activated carbon,and densities of microspore on the rate of microspore embryogenesis were analyzed.The results showed that genotypes had a significant impact on the success of microspore culture in mustard,with 11 out of 34 inbred lines successfully producing em-bryoids.There were significant differences in rates of producing embryo among different inbred lines,among which Brassica juncea var.megarrhiza Tsen et Lee had the highest rate of producing embryo,reaching 23.85 embryos/anther.The optimal conditions for producing microspores in mustard were 32℃heat shock for 1-2 days,culture medium containing 3-5 g/L activated carbon,and the density being 1.5×105 to 2.0×105 microspores/mL.The results of chromosome doubling experiment showed that immersing the stem tips in 1 g/L colchicine solution for 1 hour had a high efficiency of chromosome doubling and low chimerism,which could be used to double the chromosomes in mustard.The results of chromosome doubling test showed that 1 g/L colchicine solution soaked in stem tip for 1 hour had high doubling efficiency and less mixed-ploidy formation,which could be used to double the chromosomes in mustard.
关键词
芥菜/小孢子培养/双单倍体/小孢子胚胎发生/染色体加倍Key words
mustard/microspore culture/doubled haploid/microspore embryogenesis/chromosome doubling引用本文复制引用
基金项目
中央高校基本科研业务费专项(2662021YLQD004)
国家自然科学基金(32072573)
国家自然科学基金(32172601)
芥菜苔优质多抗新品种培育与推广项目(HBZY2023B004-4-4)
贵州省科技支撑计划(黔科合支撑[2022]一般086)
出版年
2024
NETL
NSTL
万方数据