Inhibition effect of oxidized graphene based two-dimensional composite nanosheets on porcine epidemic diarrhea virus
Two-dimensional composite nanosheet materials(GO-MnO2 NSs)were prepared by com-bining graphene oxide(GO)with MnO2 nanosheets(MnO2 NSs)through π-π stacking force to develop new broad-spectrum nanomaterials for anti-animal borne virus infections.The morphology,structure,crys-tallinity and element valences of GO-MnO2 NSs were characterized by TEM,XRD,XPS.The cytotoxici-ty of GO-MnO2 NSs was tested by MTT.The results showed that the cell survival rate of 625 μg/mL GO-MnO2 NSs exceeded 85%.Porcine epidemic diarrhea virus(PEDV)was used as a model virus for coronavirus to study the effect of GO-MnO2 NSs on PEDV infection through concentration for 50%of maximal effect(EC50),indirect immunofluorescence and western blot.The results showed that GO-MnO2 NSs had significant antiviral activity.It is indicated that GO-MnO2 NSs can eliminate the overexpression of reactive oxygen species caused by viral infection and inhibit cell apoptosis,while upregulating the expres-sion of antiviral factors including interferon and interferon stimulating genes(ISGs)in cells as well,effec-tively inhibiting viral infection.
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