氧化石墨烯基二维复合纳米片对猪流行性腹泻病毒的抑制作用研究
Inhibition effect of oxidized graphene based two-dimensional composite nanosheets on porcine epidemic diarrhea virus
李星星 1左涵琪 1李春巧 1杜婷 1杜欣军1
作者信息
- 1. 天津科技大学食品科学与工程学院/食品营养与安全国家重点实验室,天津 300457
- 折叠
摘要
为研发抗动物源性病毒感染的新型广谱纳米材料,将氧化石墨烯(GO)与二氧化锰纳米片(MnO2 NSs)通过π-π堆叠作用制备合成二维复合纳米片材料(GO-MnO2 NSs),分别通过TEM、XRD、XPS对GO-MnO2 NSs的形貌、结构、结晶度和元素价态进行表征,并采用MTT法对GO-MnO2 NSs的细胞毒性进行测试.结果显示,625 μg/mL GO-MnO2 NSs的细胞存活率超过85%;以猪流行性腹泻病毒(PEDV)作为冠状病毒的模式病毒,EC50、间接免疫荧光和Western blot试验结果证实GO-MnO2 NSs对PEDV感染具有显著的抗病毒活性.研究结果表明,GO-MnO2 NSs能够清除由病毒感染引起的活性氧过表达并抑制细胞凋亡,同时还能上调细胞内干扰素和干扰素刺激基因(ISGs)等抗病毒因子的表达,从而有效抑制病毒感染.
Abstract
Two-dimensional composite nanosheet materials(GO-MnO2 NSs)were prepared by com-bining graphene oxide(GO)with MnO2 nanosheets(MnO2 NSs)through π-π stacking force to develop new broad-spectrum nanomaterials for anti-animal borne virus infections.The morphology,structure,crys-tallinity and element valences of GO-MnO2 NSs were characterized by TEM,XRD,XPS.The cytotoxici-ty of GO-MnO2 NSs was tested by MTT.The results showed that the cell survival rate of 625 μg/mL GO-MnO2 NSs exceeded 85%.Porcine epidemic diarrhea virus(PEDV)was used as a model virus for coronavirus to study the effect of GO-MnO2 NSs on PEDV infection through concentration for 50%of maximal effect(EC50),indirect immunofluorescence and western blot.The results showed that GO-MnO2 NSs had significant antiviral activity.It is indicated that GO-MnO2 NSs can eliminate the overexpression of reactive oxygen species caused by viral infection and inhibit cell apoptosis,while upregulating the expres-sion of antiviral factors including interferon and interferon stimulating genes(ISGs)in cells as well,effec-tively inhibiting viral infection.
关键词
氧化石墨烯/二氧化锰纳米片/猪流行性腹泻病毒/抗病毒活性/纳米治疗平台Key words
graphene oxide(GO)/MnO2 nanosheets(MnO2 NSs)/porcine epidemic diarrhea virus(PEDV)/antiviral activity/nano-therapy platform引用本文复制引用
出版年
2024
NETL
NSTL
万方数据