类枯草杆菌蛋白酶基因GmSBT1在大豆共生固氮中的功能
Function of subtilases GmSBT1 in symbiotic nitrogen fixation of soybean
雷思杰 1黄诗宸 1李友国 1林会1
作者信息
- 1. 农业微生物资源发掘与利用全国重点实验室/华中农业大学生命科学技术学院,武汉 430070
- 折叠
摘要
为研究SBT(subtilases)家族蛋白在豆科植物共生固氮中的作用机制,分别利用生物信息学分析、时空表达定位、GUS 染色定位和基因沉默等方法来研究GmSBT1在大豆-根瘤菌共生中的作用.结果显示:GmSBT1受根瘤菌特异性诱导,在成熟根瘤中高量表达,且在根瘤皮层以及含菌细胞中发挥功能.GmSBT1-RNAi植株地上鲜质量、瘤质量、固氮酶活显著降低.结果表明,GmSBT1 蛋白对根瘤的形成和发育以及根瘤的固氮具有重要的作用.
Abstract
Bioinformatics analysis,spatiotemporal expression localization,GUS staining localization and gene silencing were used to study the mechanism of SBT family proteins in the soybean-rhizobia symbi-osis to investigate the function of the GmSBT1 gene in symbiotic nitrogen fixation between rhizobia and host plants.The results showed that the GmSBT1 gene was specifically induced by rhizobia and only ex-pressed at high levels in the nodules,possibly functioned in the rhizobia cortex and bacterial cells.RNA in-terference significantly reduced the fresh weight,nodule weight,and nitrogenase activity aboveground.It is indicated that the GmSBT1 protein plays an important role in the formation and development of nodules,and the nitrogen fixation in nodules as well.
关键词
蛋白酶/类枯草杆菌蛋白酶/根瘤菌/共生固氮/大豆Key words
protease/subtilizes/rhizobium/symbiotic nitrogen fixation/soybean引用本文复制引用
出版年
2024
NETL
NSTL
万方数据