摘要
为探究烤烟连作障碍的关键影响因素及规律,对汉中地区种植5种不同年份处理:连作0 a(CK)、连作2 a(T1)、连作4 a(T2)、连作6 a(T3)和连作8 a(T4)的烤烟土壤进行了土壤理化性质、微生物多样性及群落结构变化的分析.结果显示:烤烟土壤有机质、土壤pH和总碳含量随着种植年限的增加而降低;T4处理相比于CK处理,土壤有机质、土壤pH和总碳含量分别减少8.34%、25.39%和25.20%;土壤中细菌和真菌多样性及丰富度均呈现先增加后减少的趋势,其中细菌优势菌门为放线菌门、变形菌门、酸杆菌门、绿弯菌门和厚壁菌门;真菌优势菌门为子囊菌门、担子菌门和毛霉菌门,相比于CK处理,T3处理下微生物相对丰度变化较大,其中放线菌门、厚壁菌门和担子菌门变化幅度分别为4.04%、6.24%和28.86%.结果表明,连作降低了土壤有机质含量、土壤pH和全碳含量,影响土壤微生物群落组成.
Abstract
The changes in the physicochemical properties,the diversity of microorganisms and the structure of microbial community in the soil planted continuously with flue-cured tobaccos of different plant-ing years including continuous cropping for 0 year(CK),continuous cropping for 2 years(T1),continu-ous cropping for 4 years(T2),continuous cropping for 6 years(T3),and continuous cropping for 8 years(T4)in Hanzhong area of Shaanxi Province were analyzed to study the key factors affecting the obstacles for continuous cropping of flue-cured tobacco and its patterns.The results showed that the organic matter,pH,and the content of total carbon in soil decreased with the increasing years of planting.The organic mat-ter,pH,and the content of total carbon in the soil of T4 was 8.34%,25.39%,and 25.20%lower than that of CK.The diversity and abundance of bacteria and fungi in soil showed a trend of increasing first and then decreasing,with the dominant bacterial phyla being Actinobacteriota,Proteobacteria,Acidobacterio-ta,Chloroflexi and Firmicutes.The dominant fungal phyla were Ascomycota,Basidiomycota,and Mu-coromycota.Compared with CK,the relative abundance of microorganisms under T3 changed significant-ly,with change in Actinobacteria,Firmicutes,and Basidiomycota of 4.04%,6.24%,and 28.86%,re-spectively.It is indicated that continuous cropping of flue-cured tobaccos reduces the content of organic mat-ter,pH,and the content of total carbon in soil,affecting the composition of microbial community in soil.
基金项目
国家重点研发计划项目(2021YFD1700900)
汉中烟草科技攻关项目(2021HZ05)
NETL
NSTL
万方数据