Biocontrol potential and genomic analysis of salt tolerant bacterium Bacillus sp.SF-18
The dilution coating plate method was used to isolate and screen a salt-tolerant strain SF-18 with significant antibacterial activity from soil samples in Aksu saline alkali land,Xinjiang to obtain bio-control agents with elite antibacterial activity from extreme environments.The stability of the antagonistic activity of the supernatant of strain SF-18 cell-free fermentation was determined using the Oxford Cup method,with the Verticillium dahliae as an indicator strain.The whole genome of strain SF-18 was se-quenced with Illumina Novaseq platform and PacBio sequel platform.Data sequenced were used to assem-bly gene,annotate the function of gene,and predict gene clusters involved in synthesizing secondary metabo-lites.The results showed that strain SF-18 had a significant inhibitory effect on Staphylococcus aureus and Verticillium dahliae,and grew in the broth containing 18%NaCl.The supernatant of strain SF-18 cell-free fermentation had strong antibacterial activity at-20-80℃and tolerated pH 5-12 environments.The strain SF-18 was identified as belonging to the genus Bacillus based on analyzing the sequence of 16S rD-NA gene and gyrB gene.The results of whole genome analysis showed that there were 480 genes of strain SF-18 involved in the metabolism of multiple carbon sources,including genes encoding compounds related to stress resistance such as spermidine and trehalose,and genes related to enzymes such as glucanase and chitinase that can hydrolyze the cell wall of pathogen.The results of predicting secondary metabolites showed that strain SF-18 contained gene clusters involved in synthesizing a various of resistant compounds including bacillaene,bacillibactin,mersacidin,fengycin,zwittermicin.It is speculated that strain SF-18 may achieve antibacterial effects by producing antibacterial secondary metabolites and related degradation en-zymes,and has good application prospects in biological control of agriculture.
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