摘要
为了初步揭示玉米粗蛋白含量、淀粉含量、中性洗涤纤维(neutral detergent fiber,NDF)含量、酸性洗涤纤维(acid detergent fiber,ADF)含量、可溶性糖含量和体外干物质消化率等性状的遗传规律,按照随机区组设计将183份自交系为材料种植于贵阳,测定玉米粗蛋白含量、淀粉含量、NDF含量、ADF含量、可溶性糖含量和体外干物质消化率,利用Maize SNP 50芯片对供试材料进行基因分型,采用混合线性模型进行全基因组关联分析,结果显示,分别鉴定出31、61、11、36、20和42个与粗蛋白、淀粉、NDF、ADF、可溶性糖及体外干物质消化率显著相关的单核苷酸多态性位点(SNP)(P<0.001),对表型变异的解释率分别为5.80%～11.40%、5.78%～11.38%、5.78%～7.85%、5.81%～10.37%、5.78%～7.35%和 5.79%～11.33%.同 时,发 现 SYN6712、PHM1190.3、SYN7541和PZE-104072386属于一因多效位点;其中,6号染色体上的SYN6712、PHM1190.3和SYN7541同时与淀粉、体外干物质消化率显著关联,4号染色体上的PZE-104072386同时与ADF、可溶性糖显著关联,经过等位变异鉴定发现T/T基因型是SYN6712 和PHM1190.3 的优异等位变异,并挖掘到了Zm00001d037272、Zm00001d037386、Zm00001d037532和Zm00001d051166等候选基因.
Abstract
183 maize inbred lines were planted in Guiyang according to a random block design to pre-liminarily study the genetic patterns of the content of crude protein,starch,neutral detergent fiber(NDF),acid detergent fiber(ADF),soluble sugar,and the in vitro digestibility of dry matter in maize.The content of crude protein,starch,NDF,ADF,soluble sugars,and the in vitro digestibility of dry matter in maize was determined.The Maize SNP 50 chip was used to genotype materials tested.Genome-wide association analysis(GWAS)with the mixed linear model(MLM)was performed.The results showed that 31,61,11,36,20,and 42 single nucleotide polymorphisms(SNPs)sites were identified to be significantly associated with the content of crude protein,starch,NDF,ADF,soluble sugars,and the in vitro digestibility of dry matter in maize(P<0.001),with the explanation rates for phenotypic variation of 5.80%-11.40%,5.78%-11.38%,5.78%-7.85%,5.81%-10.37%,5.78%-7.35%,and 5.79%-11.33%,respectively.It was found that SYN6712,PHM1190.3,SYN7541,and PZE-104072386 belong to a pleio-tropic locus.Among them,SYN6712,PHM1190.3,and SYN7541 on the chromosome 6 were significant-ly associated with the content of starch and the in vitro digestibility of dry matter,while PZE-104072386 on the chromosome 4 was significantly associated with the content of ADF and soluble sugars.The results of allele identification showed that the T/T genotype was an elite allele of SYN6712 and PHM1190.3,and candidate genes including Zm00001d03772,Zm00001d037386,Zm00001d037532,and Zm00001d051166 were identified.It will provide theoretical support for the further studies on the genetic mechanism of quality related traits in maize including the content of crude protein,starch,NDF,ADF and soluble sugars,as well as for the molecular markers assisted breeding.
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