'东试早柚'果实无核成因探究
Studies on seedlessness mechanism in'Dongshi Zaoyou'pummelo
刘承浪 1黄文铠 2闫素云 3周先艳 3董美超 3徐祥增 4高世德 4高俊燕 3柴利军 2邓秀新2
作者信息
- 1. 果蔬园艺作物种质创新与利用全国重点实验室/华中农业大学园艺林学学院,武汉 430070;云南省农业科学院热带亚热带经济作物研究所,保山 678000
- 2. 果蔬园艺作物种质创新与利用全国重点实验室/华中农业大学园艺林学学院,武汉 430070
- 3. 云南省农业科学院热带亚热带经济作物研究所,保山 678000
- 4. 云南省热带作物科学研究所,景洪 666100
- 折叠
摘要
为明确'东试早柚'果实无核成因,以'东试早柚'及其杂交群体为试材,利用醋酸洋红和苯胺蓝染色法、杂交授粉及分子标记等手段探究'东试早柚'无核原因.结果显示:'东试早柚'的花结构完整,花粉活力及萌发率分别为94.80%和86.20%;'东试早柚'自交表现为不亲和,而与'沙田柚'杂交亲和;利用S5、S6基因型分子标记从'沙田柚'×'东试早柚'的175株杂交子代中共鉴定出F1代杂种164株,排除了'东试早柚'雄性不育的可能;连续2 a田间试验观察显示,'东试早柚'刺激性单性结实和天然单性结实的平均座果率分别为13.04%和25.56%.结果表明,'东试早柚'自交不亲和并具有一定的单性结实能力是导致其无核的主要成因.
Abstract
'Dongshi Zaoyou'pummelo and its hybrid offsprings,methods including magenta acetate staining and aniline blue staining,cross-pollination,and molecular markers were used to study the mecha-nism of seedlessness in'Dongshi Zaoyou'pummelo.The results showed that the floral structure of'Dong-shi Zaoyou'pummelo was intact,with pollen vitality and germination rates of 94.80%and 86.20%,respec-tively.The self-pollination of'Dongshi Zaoyou'pummelo showed incompatibility,while cross-pollination with'Shatianyou'pummelo showed compatibility.A total of 164 F1 hybrids were identified from 175 hy-brid offsprings of'Shatianyou'pummelo×'Dongshi Zaoyou'pummelo with S5/S6 molecular marker ge-notyping,ruling out the possibility of male sterility in'Dongshi Zaoyou'pummelo.The results of observa-tion for two consecutive years showed that the mean fruit setting rates of stimulating parthenocarpy and nat-ural parthenocarpy in'Dongshi Zaoyou'pummelo was 13.04%and 25.56%,respectively.It is indicated that the main reason for the seedless development of'Dongshi Zaoyou'pummelo is its self-incompatibility and certain ability to produce parthenocarpy.
关键词
'东试早柚'/雄性不育/自交不亲和/单性结实Key words
'Dongshi Zaoyou'pummelo/male sterility/self-incompatibility/parthenocarpy引用本文复制引用
出版年
2024
NETL
NSTL
万方数据