Studies on seedlessness mechanism in'Dongshi Zaoyou'pummelo
'Dongshi Zaoyou'pummelo and its hybrid offsprings,methods including magenta acetate staining and aniline blue staining,cross-pollination,and molecular markers were used to study the mecha-nism of seedlessness in'Dongshi Zaoyou'pummelo.The results showed that the floral structure of'Dong-shi Zaoyou'pummelo was intact,with pollen vitality and germination rates of 94.80%and 86.20%,respec-tively.The self-pollination of'Dongshi Zaoyou'pummelo showed incompatibility,while cross-pollination with'Shatianyou'pummelo showed compatibility.A total of 164 F1 hybrids were identified from 175 hy-brid offsprings of'Shatianyou'pummelo×'Dongshi Zaoyou'pummelo with S5/S6 molecular marker ge-notyping,ruling out the possibility of male sterility in'Dongshi Zaoyou'pummelo.The results of observa-tion for two consecutive years showed that the mean fruit setting rates of stimulating parthenocarpy and nat-ural parthenocarpy in'Dongshi Zaoyou'pummelo was 13.04%and 25.56%,respectively.It is indicated that the main reason for the seedless development of'Dongshi Zaoyou'pummelo is its self-incompatibility and certain ability to produce parthenocarpy.