猪精液冻融技术及其影响因素研究进展
Research progress on porcine semen cryopreservation technology and its influencing factors
范振港 1刘鑫 1苗义良 2张霞1
作者信息
- 1. 华中农业大学动物科学技术学院、动物医学院/农业动物遗传育种与繁殖教育部重点实验室,武汉 430070
- 2. 华中农业大学动物科学技术学院、动物医学院/农业动物遗传育种与繁殖教育部重点实验室,武汉 430070;动物育种与健康养殖教育部前沿科学中心,武汉 430070
- 折叠
摘要
养猪业是我国畜牧产业的重要组成部分.国内生猪养殖主要采用自繁自养模式,配种所使用的精液多采用鲜精或常温稀释保存精液,而精液冻融技术可实现猪精液的长期保存,但与牛、小鼠等物种相比较,猪精液冻融技术还有待于进一步优化改善.为进一步改进猪精液冷冻技术并实现在生产上大规模应用,本文主要综述了猪精液冻融技术发展概况、主要技术流程,影响猪精液冻融后精子活力的关键因素,包括精液的采集、冷冻前降温平衡、冷冻保护剂添加和冷冻方法等,以及精子冻融过程中的理化性质变化,包括物理结构损伤、功能性损伤、精子抗冻机制等,最后总结、展望了能够提高猪精液冻融效果的新方法.
Abstract
Pig industry is an important component of animal husbandry industry in China.The domes-tic pig production mainly adopts self-breeding mode.The semen used in pig reproduction is usually fresh se-men or diluted at room temperature,while semen cryopreservation technology can realize the long-term preservation of pig semen.Compared with cattle,mice and other species,the cryopreservation technology of pig semen needs to be further optimized and improved.In order to provide a theoretical reference for per-fecting the freezing technique of pig semen and accelerating its large-scale application in pig production,this review summarized the development and main technological process of freezing and thawing of porcine se-men,and the key factors affecting sperm motility after freezing and thawing,including semen collection,pre-cooling balance before freezing,addition of cryoprotectant agents,and cryopreservation methods.It also covered the physicochemical changes during the sperm cryopreservation process,such as physical structural damage,functional damage,and sperm anti-freezing mechanisms.Finally,the new methods to improve the cryopreservation efficiency of pig sperm were summarized and prospected,including nanotechnology treat-ment of semen and the study of cryopreservation biomarkers for sperm.
关键词
猪/精液冻融/冷冻保护剂/冻精解冻剂/精液理化性质Key words
pig/frozen semen thawing/cryoprotectant/thawing extender additive/physical and chemical properties of sperm引用本文复制引用
出版年
2024
NETL
NSTL
万方数据