Elimination effect of SEYT4 on common pathogenic bacteria in food chain
In order to explore the application prospect of phage-derived peptide in food processing chain,SEYT4,a novel phage-derived peptide containing 37 amino acids was selected as the research ob-ject and its structural characteristics were analyzed.The common pathogenic bacteria in food processing chain were selected to carry out bactericidal tests under different temperature,salinity and pH to determine the bactericidal spectrum,bactericidal efficiency and bactericidal characteristics of SEYT4.The safety of SEYT4 was evaluated in C57BL/6 female mice,and the efficacy of SEYT4 in removing pathogenic bacte-ria from the food chain was investigated by simulating pathogen contamination in leafy vegetables and kitch-enware.The results showed that:SEYT4 contained a hairpin shaped double α-helical structure with a mo-lecular mass of about 4.5 ku and a theoretical isoelectric point of 9.69.It could eliminate most of the highly toxic and drug-resistant Gram-positive and Gram-negative bacteria with a bactericidal rate as high as 99.99%.It had high activity at different temperatures,and the bactericidal effect was better in neutral and weak alkaline environment but inactivated in high salt environment.There was no hemolysis effect on mice erythrocytes.In the artificial pollution model of coriander,pathogenic bacteria in leaves and washing buffer could be removed about 90.00%,and more than 99.00%of pathogenic bacteria on food contact surface could be removed.The results showed that bacteriophage peptide SEYT4 was a novel antimicrobial peptide with good bactericidal effect and high safety,which could effectively prevent and control the pathogenic bac-teria contaminated in food chain and was expected to be a practical disinfectant in food processing chain.
NETL
NSTL
万方数据
