Analysis of the Expression Patterns of Creatine-related Genes in the Mouse Central Nervous System
Creatine is able to maintain adenosine triphosphate(ATP)balance in cells and plays a key role in cellular energy metabolism.Abnormal synthesis and transport of creatine can lead to creatine deficiency syndrome(CDS),resulting in neurodevelopmental delay and neurodegenerative diseases.To date,the expression patterns of creatine-related genes in the central nervous system(CNS)remain poorly identified.By screening the transcriptome database,it was initially found that creatine related genes Slc6a8,Gatm and Gamt were predominantly expressed in oligodendrocyte(OL)lineage cells.To test this finding,the expression patterns of Slc6a8,Gatm and Gamt in mouse brain and spinal cord tissues at different developmental stages were examined through RNA in situ hybridization.Immunohistochemistry confirmed that Slc6a8,Gatm and Gamt were mainly expressed in differentiated oligodendrocytes.The results suggest that oligodendrocytes may be the major creatine synthesizing cells in the CNS.Oligodendrocytes play an important role in the maintenance of creatine homeostasis and energy metabolism in the whole central nervous system.
国家科技期刊平台
NSTL
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