牡丹茄腐镰刀菌FSH基因组中GATA类转录因子的鉴定与分析
Identification and Analysis of GATA-Type Transcription Factors in the FSH Genome of Fusarium solani On Paeonia
袁琴琴 1晏思雨 1仝笑笑 1贾媛丹1
作者信息
- 1. 菏泽学院农业与生物工程学院(牡丹学院),山东 菏泽 274015
- 折叠
摘要
牡丹根腐病主要是由茄腐镰刀菌(Fusarium solani)引起的,对牡丹种植区危害很大.GATA转录因子在病原真菌的定殖和致病过程中发挥重要作用.在牡丹茄腐镰刀菌 FSH 基因组中共鉴定出 6 条GATA类转录因子,它们均定位于细胞核中.在这 6 条 GATA 类转录因子编码的蛋白质中,有 4 个碱性蛋白(g2237.t1,g4943.t1,g6316.t1 和g677.t1),1 个酸性蛋白(g3670.t1)和 1 个中性蛋白(g13425.t1).这 6个蛋白质均为亲水性和不稳定蛋白,它们编码的氨基酸数目区间为 435～1025.保守结构域分析发现,这 6个蛋白质除含有ZnF-GATA结构域外,g13425.t1 还编码两个 PAC 结构域,g13425.t1 和g3670.t1 编码有PAS结构域;三级结构预测显示这 6 个蛋白质均无信号肽序列.研究阐述了牡丹茄腐镰刀菌FSH 基因组中的GATA类转录因子基因及其生物信息学特征,以期为后期研究该类转录因子的功能提供一定的理论基础.
Abstract
Root rot in Paeonia is mainly caused by Fusarium solani,which poses a significant threat to peony-growing areas.GATA transcription factors play a crucial role in colonization and pathogenicity in pathogenic fungi.In the FSH genome of Fusarium solani associated with Paeonia root rot,6 GATA tran-scription factors were identified,all localized in the nucleus.Among these 6 GATA transcription factors,4 are basic proteins(g2237.t1,g4943.t1,g6316.t1,g677.t1),1 is acidic(g3670.t1),and 1 is neutral(g13425.t1).All these proteins are hydrophilic and unstable,with the number of amino acids ranging from 435 to 1025.Conserved domain analysis revealed that these proteins contain the ZnF-GATA domain,with g13425.t1 also encoding two PAC domains,and both g13425.t1 and g3670.t1 containing PAS do-mains.Tertiary structure prediction indicated that none of these proteins have signal peptide sequences.The study explains the genomic GATA transcription factors and their bioinformatics characteristics in Fu-sarium solani associated with Paeonia,providing a theoretical basis for further research on the functional-ity of this class of transcription factors.
关键词
牡丹/茄腐镰刀菌/基因组/GATA转录因子/鉴定Key words
paeonia/fusarium solani/genome/GATA transcription factors/identification引用本文复制引用
出版年
2024
NETL
NSTL
万方数据