Effect of different carbon sources on growth of thermoresistant Yarrowia lipolytica
[Objective]The effect of different carbon sources was investigated on the growth of thermoresistant Yarrowia lipolytica and erythritol synthesis to improve the production of erythritol from thermoresistant Yarrowia lipolytica by optimizing the combination of carbon sources.[Method]Culture media containing different carbon sources were used to cultivate the Y.lipolytica CA20,Y.lipolytica HT34,and Y.lipolytica HT38 grown at 30 ℃,34 ℃,and 38 ℃,respectively,during which the optical density of cell culture was detected at a wave length of 600 nm(OD600)with an interval of 24 h.Meanwhile,the concentrations of erythritol and glucose were assayed by using high performance liquid chromatography(HPLC).[Result]Strain CA20 is able to use such carbon sources as glucose,fructose,galactose,arabinose,xylose,maltose,sucrose,cellobiose,mannitol,sorbitol,glycerol,soluble starch,and soybean oil to grow,among which glucose,fructose,mannitol,glycerol,and soybean oil are the optimal carbon sources;the growth of HT34 is inhibited when using carbon sources except for glucose and fructose,while HT38 can only use glucose and soybean oil to support its growth;the addition of glucose and another one carbon source exerts little effect on the growth of strain CA20,while the addition of glucose and soybean oil promotes the growth of HT34 and HT38 obviously.In addition,the addition of soybean oil can significantly improve the erythritol production and yield by HT34 and HT38,with an increase as much as 50％.[Conclusion]These findings can provide a basis for the erythritol production using high temperature fermentation.
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