Investigation on infection of severe fever with thrombocytopenia syndrome virus in two animals in Shangcheng County,Henan Province
Objective To clarify the risk and influential factors of severe fever with thrombocytopenia syndrome virus(SFTSV)transmission in natural foci of severe fever with thrombocytopenia syndrome(SFTS)by conducting an investigation and research on the infection of animal hosts in natural foci of SFTS,so as to provide scientific evidence for targeted prevention and control of SFTS.Methods Satellite positioning technology was used to continuously track the daily activities of domestic dogs in Qiuwan,Pingtang and Longtouqiao villages in Shangcheng County during two weeks of monitoring window.Serum samples of dogs were collected and detected SFTSV-IgG antibody by using indirect immunofluorescence assay(IFA)method.Multivariable logistic regression model was applied to analyze the effect of environmental influential factors for infection with SFTSV in domestic dogs.The species of rodents and small mammals in the active area of domestic dogs were investigated by night trapping,night-cage and sticky board methods.The conserved genes were identified by PCR.The SFTSV infection was detected by real-time PCR and enzyme linked immunosorbent assay(ELISA).Results The daily activity range of 26 domestic dogs was tracked by satellite positioning technology,and 52 serum samples were collected from 37 domestic dogs.The total positive rate of SFTSV antibody was 59.46％.A total of 419 rodents and small mammals were captured,and the dominant rat species were Rattus tanezumi,Mus musculus,and Apodemus agrarius,and no positive samples were detected for SFTSV.The longer distance from the domestic dog activity center to the main road and the higher frequency of visiting forest land were the risk factors for SFTSV infection.The longer distance from the domestic dog activity center to the forest land was the protective factor.Conclusions The positive rate of SFTSV antibody in domestic dogs in Shangcheng County is high,and the infection of SFTSV is related to the ecological environment.It is recommended to further expand the species of domestic animals for the future investigation and research on their pathogenic infection.In Shangcheng County,there is a large variety of rodent species yet no detection of SFTSV.Thus,the role of rodents in the natural cycle of SFTSV demands further investigation.
Severe fever with thrombocytopenia syndrome virusTick-borne diseasesRodentSerologyRisk factorsThrombocytopenia syndromeFever
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