Identification of immune molecular markers for ischemic stroke based on genomic data
Objective To explore the immune-related molecular markers for ischemic stroke based on genomic data,and to provide theoretical basis for the prevention and clinical treatment of ischemic stroke.Methods GSE16561 and GSE58294 from the gene expression omnibus(GEO)database were used for the analysis and exploration of molecular markers immune-related to ischemic stroke.Based on the immunology database and analysis portal(ImmPort)database,immune-related genes were obtained and their differential expression in ischemic stroke group and normal controls group were also analyzed to identify differentially expressed immune genes(DEIGs).Immune-related hub genes were identified by protein-protein interaction(PPI).Pathway analysis of DEIGs was performed to find the possible molecular signaling pathways of ischemic stroke.Finally,the infiltration abundance of 22 immune cells was evaluated based on the CIBERSORT algorithm,and the difference between ischemic stroke group and normal control group was subsequently calculated to determine ischemic stroke-related immune cells.Results Differential analysis results showed that 37 DEIGs had statistically significant differences between two groups(all P＜0.05),including 31 up-regulated genes and 6 down-regulated genes in ischemic stroke samples.Pathway analysis results showed that the identified DEIGs were mainly enriched in immune-related molecular pathways.PPI analysis results showed that TLR4,TLR2,MMP-9,CCR7,STAT3,TNFSF13B,S100A12,CD19,CAMP and SLC11A1 were key hub immune genes closely related to ischemic stroke.Based on CIBERSORT results,we found that immune response cells were less enriched in ischemic stroke samples(all P＜0.05).However,the levels of immunosuppressive cells were higher(all P＜0.05).Conclusions Based on transcriptome expression data,this study identifies immune genes,immune signaling pathways and immune cells associated with ischemic stroke.This study reveals the immune molecular markers associated with ischemic stroke at three molecular levels,which is of great significance for the effective prevention and control of ischemic stroke and the formulation of targeted treatment strategies.
Ischemic strokeImmune genesSignaling pathwaysImmune cellsMolecular markersOmics data
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