Objective To understand the current research status of clustered regularly interspaced short palindromic repeats(CRISPR)applied to nucleic acid detection.Methods In this study,we systematically reviewed related literatures published on databases of PubMed,BioRxiv,and MedRxiv using the keywords"CRISPR","nucleic acid detection","point-of-care testing"or"POCT".Results According to the inclusion and exclusion criteria,a total of 17 publications were selected.We summarized and analyzed the information of CRISPR-associated protein,amplification method,sensitivity,specificity,detection time and other aspects.Results suggested that when combined with nu-cleic acid amplification methods,such as loop-mediated isothermal amplification(LAMP)or recombinase polymerase amplification(RPA),the sensitivity of CRISPR was comparable to real time quantitative polymerase chain reaction(RT-qPCR).Conclusions The process of CRISPR can be completed within 30 to 100 minutes,with results that can be read by the naked eye.Additionally,the cost of detection is comparable to the antigen test,making it particularly suitable for large-scale point-of-care(POC)nucleic acid detection in resource limited areas.
关键词
成簇规律间隔短回文重复序列/核酸检测/即时检测
Key words
Clustered regularly interspaced short palindromic repeats/Nucleic acid detection/Point-of-care testing
维普
万方数据