Associative Effects of Cornstalk, Wheat Straw and Corn Stalk Silage by Gas Production Technique in Vitro
This research was to investigate the associative effects of cornstalk (CS),wheat straw (WS),and corn stalk silage (CSS) by gas production technique in vitro.Single factor experiment was designed to begin with CS and WS to screen for the best combination of CS-WS,followed by a second screen for the best combination of CS-WS-CSS with the best combination of CS-WS and CSS.All combinations above were tested with the ratios of 100:0,80:20,60:40,50:50,40:60,20:80 and 0:100,with 3 replicates.Gas production (GP),rate of dry matter loss,pH,microbial crude protein (MCP),ammonia nitrogen (NH3-N),as well as their single factor associative effects index (SFAEI) and multiply factors associative effects index (MFAEI) were evaluated.The results are shown as follows: Different combinations showed significant differences (P<0.05 or P<0.01) on the rate of gas production.To produce the best SFAEI of gas rate,the optimal ratio for CS and WS was 60:40,and the best combination of CS-WS and CSS was 40:60;Significant differences (P<0.05 or P<0.01) were detected for dry matter loss rate between different combinations,and the best SFAEI of dry matter loss was the combination of 50:50 and 20:80,respectively.There was no significant difference on pH between different feed combinations (P>0.05).MCP yield was extremely significantly (P<0.01) different between the two feed combinations.To be specific,MCP decreased when increasing WS proportion,while increased when CSS proportion increased,and the best SFAEI of MCP was the combination of 60:40 and 40:60,respectively.NH3-N concentration was significantly different (P<0.05) between different feed combinations,changing from 10.71 to 26.05 mg/dl,but they were within the normal range.Using MFAEI to assess the optimal ratios of associative effects,CS and WS were 60:40,the best combination of CS-WS (60:40) and CSS were 20:80.Overall,the best combination of CS-WS-CSS was 12:8:80.
in vitrocornstalkwheat strawcorn stalk silageassociative effects
国家科技期刊平台
NSTL
万方数据
维普
