不同青贮饲料对奶牛瘤胃液微生物多样性的影响
Effects of Different Silage on Microbial Diversity in Rumen Fluid of Dairy Cows
周斐然 1赵龙飞 1张梦圆 1张苏江2
作者信息
- 1. 哈密市畜牧工作站,新疆哈密 839000
- 2. 塔里木大学动物科学与技术学院,新疆阿拉尔 843300
- 折叠
摘要
为了更好地开发利用甜高粱青贮饲料,采用Illumina MiSeq 2 × 250系统,对细菌16S rDNA V3～V4区进行高通量测序,分析了分别饲喂高糖甜高粱青贮(HSS)、低糖甜高粱青贮(LSS)和玉米青贮(MZS)3种青贮饲料奶牛瘤胃液样本中的细菌多样性及群落结构.结果表明:HSS组和MZS组瘤胃液样品中菌类丰富度相近且高于LSS组;HSS组、MZS组中拟杆菌门(Bacteroidetes)所占比例最大,极显著高于LSS组(P＜0.05),分别占39.65％和40.51％;其次是厚壁菌门(Firmicutes),分别占29.67％和32.87％,低于LSS组;通过菌属的分析,普雷沃菌属(Prevotella)和瘤胃球菌属(Ruminococcus)在3种瘤胃液中都是优势菌群,HSS组与MZS组瘤胃液中的细菌菌群有许多共性,且菌属丰富度高于LSS组.结果提示HSS可代替MZS用于饲喂奶牛.
Abstract
In order to better exploit and utilize sweet sorghum silage,Illumina MiSeq 2 × 250 system was used to perform high-throughput sequencing of the V3-V4 region of bacterial 16SrDNA.The bacterial diversity and community structure of rumen fluid samples from high sugar sweet sorghum silage(HSS),low sugar sweet sorghum silage(LSS)and maize silage(MZS)were analyzed.The results showed that the microbial richness of rumen fluid extracted from HSS and MZS was similar and higher than in LSS.The proportion of Bacteroidetes in HSS and MZS was the largest,which was significantly higher than that in LSS(P＜0.05),accounting for 39.65％and 40.51％,respectively.Firmicutes accounted for 29.67％and 32.87％,which were lower than in LSS.According to the analysis of bacterial genera,Prevotella and Ru-minococcus were dominant bacteria in three rumen fluids.There are many commonalities between HSS and MZS rumen fluids,and the richness of bacterial genera is higher than that of LSS.The results showed that HSS can be used to replace MZS to feed cows.
关键词
高通量测序/瘤胃微生物/甜高粱/多样性/奶牛Key words
high-throughput sequencing/rumen microbes/sweet sorghum/diversity/cows引用本文复制引用
基金项目
国家自然科学基金项目(31160472)
国家自然科学基金项目(31260565)
哈密市科学技术项目(hm2021kj10)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据