河南省某兔场兔肝球虫和大肠杆菌混合感染情况及耐药性研究
Study on Mixed Infection and Drug Resistance of Rabbit Coccidia and Escherichia coli in a Rabbit Farm in Henan Province
张昕 1张自强 1朱欣妍 1张晨杰 1刘玉梅1
作者信息
- 1. 河南科技大学动物科技学院,河南洛阳 471000
- 折叠
摘要
兔肝球虫和大肠杆菌是家兔养殖中常见且危害较大的疾病.为了确定河南省某兔场家兔腹泻并大规模死亡的病原,实验室对兔场发病兔进行病理剖检和寄生虫检测,采集组织进行病原菌的分离鉴定及药敏试验.结果显示:发病兔粪样检测到的球虫数量较少,未达到肠球虫感染标准;病理剖检发现个别兔肝脏有白色条形结节状结构;所有剖检兔胆汁镜检可见大量球虫卵囊;细菌分离培养得到一株革兰氏阴性杆状可疑致病菌,其eae基因序列扩增片段为833 bp,与大肠杆菌MN208178.1相似性为99.65％;经药敏测定发现分离得到的大肠杆菌同时对多黏菌素B、丁胺卡那、氨苄西林、头孢哌酮4种药物敏感,对其他药物有不同程度的耐药性.根据以上试验结果,导致此次兔场大规模发病死亡的主要原因是肝球虫和其继发的大肠杆菌混合感染.研究结果可为家兔养殖过程中肝球虫和大肠杆菌混合感染的诊断以及临床用药提供参考.
Abstract
Rabbit hepatic coccidia and Escherichia coli are common and harmful diseases in rabbit feed-ing.In order to determine the pathogens resulting in rabbits diarrhea and mass death at a rabbit farm in Henan Province,the laboratory carried out pathological autopsy,parasite detection,tissue collection for i-solation and identification of pathogens and drug sensitivity test.The results showed that the number of coccidia detected in the feces of infected rabbits was less,which did not meet the standard of intestinal coc-cidia infection.Pathological examination showed that a few rabbits'liver had white strip nodular structure.A large number of coccidia oocysts were found in bile microscopic examination of all dissected rabbits.A suspected gram-negative rod pathogen was isolated and cultured.The size of the amplified fragment of eae gene was 833 bp,and the similarity with E.coli MN208178.1 was 99.65％.It was found that the isolated Escherichia coli was sensitive to polymyxin B,amikacin,ampicillin and cefoperazone,and had different degrees of resistance to other drugs.It is concluded that the main reason of large-scale morbidity and mor-tality in the rabbit farm is the mixed infection of hepatic coccidia and its secondary Escherichia coli.The results of this study provides scientific reference for the diagnosis and clinical medication of mixed infection of hepatic coccidia and Escherichia coli in rabbits.
关键词
兔/肝球虫/大肠杆菌/耐药性Key words
rabbit/hepatic coccidia/Escherichia coli/drug resistance引用本文复制引用
基金项目
河南省重点研发与推广专项(192102110077)
河南省重点研发与推广专项(202102110093)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据