Preparation of Inactivated Vaccine of Mycoplasma synoviae and its Protective Efficacy
To study the immunoprotective effect of inactivated Mycoplasma synociae vaccine at differ-ent dosages,40 20-day-old healthy Hyland brown chicks were immunized with the inactivated vaccine made from Mycoplasma synociae strain Q5-2 in three dosage groups for three times in this study.ELISA was used to detect the serum antibody potency of each group of chickens,and after immunization,all of them were challenged through nose and eye drops to test the protective efficacy of the vaccine.The results showed that:(1)compared with the control group,the antibody titer of the three dose groups increased rapidly after the second booster immunization,in which the antibody potency of the low dose group contin-ued to rise,and the antibody potency at the peak was also the highest at 1:158,000.The difference be-tween the three dose groups was significant(P＜0.05);(2)the protection rate of the vaccine against strong venomous attack after vaccination was 100％in the low dose group,and in the middle and high dose groups it was 80％,and the probability of isolation of mycoplasma from throat swabs was significantly lower in the vaccinated group than in the control group as the time after the attack increased.It shows that the prepared inactivated vaccine against Mycoplasma synociae in chickens can stimulate the body to pro-duce a good immune response,and has a good resistance to strong virulent attack.It plays a certain inhibi-tory role in the detoxification of the animals after strong virulent infection.Thus,it provides a reference for the development of the vaccine for this disease and the research on prevention and control.
国家科技期刊平台
NSTL
万方数据
维普
