Effects of Feeding Fresh Fagopyrum dibotrys on Intestinal Microflora and Related Gene Expression of Laying Hens under High Temperature in Summer
The previous researches of the research group found that feeding fresh Fagopyrum dibotrys can maintain the laying rate of laying hens under natural high temperature conditions in summer,but the specific mechanism is unknown.This experiment intended to explore the mechanism of feeding fresh Fagopyrum dibotrys on laying hens from the perspective of intestinal related gene expression and micro-flora.300 32-week-old Changshun green shell layers were randomly divided into 5 treatment groups(Group C basic diet,T1 basic diet+5％fresh Fagopyrum dibotrys,T2 basic diet+10％Fagopyrum dibotrys,T3 basic diet+15％fresh Fagopyrum dibotrys,and T4 basic diet+20％fresh Fagopyrum dibotrys),and each treatment had 5 replicates,12 layers in each replicate,and the experimental period was 90 days.The results showed:the expression of Occluding and EGF in duodenum and ileum of laying hens in T2 significantly increased(P＜0.05).The abundance of bifidobacteria(probiotics)in the ileum and Lactobacillus in the cecum of laying hens of T2 were increased.Therefore,the supplementation of fresh Fagopyrum dibotrys to the diet of laying hens may act on the positive resistance of laying hens and allevi-ate the stress effect of high temperature environment by up-regulating the expression of Occluding and EGF genes in the intestine and increasing the abundance of beneficial bacteria in the intestine.
万方数据
维普
