为探讨角蛋白关联蛋白6。2(keratin-associated protein 6。2，KAP6。2)基因遗传多态性与内蒙古绒山羊生长性状和绒细度性状的相关性，以期为内蒙古绒山羊遗传育种改良提供理论依据，本研究采集598只雌性内蒙古绒山羊耳组织，提取基因组DNA并进行PCR扩增，检测KAP6。2基因的插入/缺失(Insertion/Deletion，InDel)突变，分析突变位点与生长性状和绒细度性状的相关性。结果显示，在育成羊中，该突变位点与胸围和十字部显著相关(P＜0。05)，与体长、胸宽和胸深极显著相关(P＜0。01);在成年羊中，该突变位点与胸宽显著相关(P＜0。05)，与胸围、十字部高和胸深极显著相关(P＜0。01);在育成羊和成年羊全部群体中，该突变位点与体长、胸围、十字部高、胸深和胸宽极显著相关(P＜0。01)，与绒细度显著相关(P＜0。05)。上述结果提示，KAP6。2基因外显子区24 bp InDel突变位点与内蒙古绒山羊部分生长性状显著或极显著相关，与绒细度显著相关，可作为内蒙古绒山羊部分生长性状和绒细度性状选育的遗传标记，为内蒙古绒山羊的选育提供科学依据。
Correlation Analysis of KAP6.2 Gene Polymorphism with Growth Traits and Cashmere Fineness in Inner Mongolia Cashmere Goats
This study aimed to evaluate the correlation between genetic polymorphisms of keratin-asso-ciated protein 6.2 gene,growth traits and cashmere fineness of Inner Mongolia cashmere goat,in order to provide a theoretical basis for genetic breeding improvement of Inner Mongolia cashmere goats.Ear tissues of 598 female Inner Mongolia cashmere goats were collected,genomic DNA was extracted and amplified by PCR to detect KAP6.2 gene Insertion/Deletion(InDel)mutation,and the correlation between mutation sites and growth traits and cashmere fineness traits was analyzed.The results showed that in the yearling goat,the mutant was significantly correlated with heart girth and height at hip cross(P＜0.05),and ex-tremely significantly correlated with body length,chest width and chest depth(P＜0.01);in the adult goat,the mutant was significantly correlated with chest width(P＜0.05)and highly correlated with heart girth and height at hip cross and chest depth(P＜0.01);in all goat population,the mutant was extremely sig-nificantly correlated with body length,heart girth and height at hip cross and chest depth and chest depth(P＜0.01),and significantly correlated with cashmere fineness(P＜0.05).The above results indicated the 24 bp InDel mutation in the exon region of KAP6.2 gene is significantly and highly significantly correlated with the partial growth traits,and significantly correlated with cashmere fineness in Inner Mongolia cashmere goat,which can be used as a genetic marker for the selection of partial growth traits and cashmere fineness traits.It provides a scientif-ic basis for the selection and breeding of Inner Mongolia cashmere goat.
万方数据
维普
