Etiological Analysis of Chicken Respiratory Diseases in Yunnan Province in 2020
To analyze the etiology of chicken respiratory disease in Yunnan Province in 2020,111 mixed samples of lungs,spleen,throat and trachea were collected from 111 chicken farms in Kunming,Honghe,Qujing,Dali,Chuxiong,and Zhaotong.PCR was preformed to detect the nucleic acids of avibacterium paragallinarum,mycoplasma gallisepticum(MG),mycoplasma synoviae(MS),newcastle disease virus(NDV),infectious bronchitis virus(IBV),infectious laryngotracheitis virus(ILTV),avian influenza vi-rus(AIV)and omithobacterium rhinotracheale(ORT).Further,some positive PCR products were veri-fied through sequencing.The results showed that positive rates of avibacterium paragallinarum,MG,MS,NDV,IBV,ILTV,ATV,and ORT were 18.92％(21/111),10.81％(12/111),5.41％(6/111),4.50％(5/111),2.70％(3/111),39.64％(44/111),9.00％(10/111),18.92％(21/111),respectively.Totally,the mixed infections accounted for 32.43％(36/111),and the mixed infection with 2,3 and 4 pathogens accounted for 18.92％(21/111),11.71％(13/111)and 1.80％(2/111),respectively.In general,the chicken respiratory disease in Yunnan Province in 2020 was caused by many factors,among which avibac-terium paragallinarum,ILTV and ORT were the main pathogens.
万方数据
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