The Effect of Proline on Goat Semen Storage at Low Temperature
To improve the antioxidant protection capacity of sperm and extend the viability of sperm in vitro,concentrations of 0,0.5,1,2,and 4 mM of proline were added to the goat semen basic dilution medium under low-temperature storage conditions(4 ℃).After 24 and 48 hours,the sperm motility,acrosome integrity,and plasma membrane integrity were evaluated in each treatment group.Additionally,mitochondrial activity,reactive oxygen species level,malondialdehyde,lipid peroxide content,antioxidant enzyme activity and other sperm quality assessment indexes were also investigated after 48 h of preservation.The results showed that addition of proline to the diluted medium improved the sperm antioxidant capacity,and the optimal concentration was 1 mM.In the 1 mM treatment group,the sperm total motility at 48 h was 71.3％,significantly increased by 18.57％compared with the control group(P＜0.01),meanwhile,acrosome integrity was increased by 12％,plasma membrane in-tegrity was increased by 10.5％,the mitochondrial activity was increased by 23.15％,and antioxidant en-zyme activity were also significantly increased(P＜0.05),while reduction of sperm ROS level,malondial-dehyde level,lipid peroxide were overserved in proline treatments(P＜0.05).Furthermore,addition of proline significantly increased the proline dehydrogenase activity(P＜0.05),it also significantly decreased the expression of the apoptosis proteins such as Caspase-3,Caspase-9,P53 and BCL-2(P＜0.05).There-fore,addition of proline to the diluted medium of goat semen preserved at 4oC improves the goat sperm quality.These findings provide novel insights that proline can serve as an effective antioxidant to improve the goat sperm quality when preserved at low temperature.
low temperature preservationprolinegoat semensemen quality
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