Polymorphism of MYF5 gene in Diannan Small-ear Pigs
Myogenic factor 5(MYF5)is an important gene affecting muscle growth and development.The purpose of this study was to investigate the effects of genetic variation of MYF5 gene on growth traits of Diannan small-ear pigs,and to provide effective molecular genetic markers for breeding new meat varie-ties(lines)of high quality Diannan small-ear pigs.Using myogenic factor 5(MYF5)as candidate gene(Duroc(n=40)as control),the dominant SNP locus of Dian-nan small-ear pigs(n=18)were screened,and the association between the selected dominant SNP and main carcass and meat quality traits were ana-lyzed.Results showed among the 16 SNP loci detected in MYF5 gene of Diannan small-ear pigs,1 is loca-ted in the coding region,and 4 dominant SNPs are screened.The results of association analysis showed that the mutation frequencies of 1486,1574,1629 and 1701 loci of MYF5 gene are consistent and the ge-netic map distance is similar,and the linkage is close.The A1486G loci were GG,AG and AA genotypes.The G1574A locus was AA,GA and GG genotypes,respectively.The C1629T loci were TT,CT and CC genotypes.The T1701C locus was CC,TC and TT genotype,respectively.The intramuscular fat content of MM genotype was significantly higher than that of MN genotype(P＜0.01),MM genotype was signifi-cantly higher than that of NN genotype(P＜0.05)and NN genotype was significantly higher than that of MN genotype(P＜0.05).In terms of marbling score,MM genotype was significantly higher than NN gen-otype(P＜0.01),MM genotype was significantly higher than MN genotype(P＜0.01),MN genotype was significantly higher than NN genotype(P＜0.01).It can be inferred that MYF5 gene is the main gene affecting meat quality traits of Diannan small-ear pigs.The results can provide molecular basis for impro-ving meat quality in breeding of Diannan small-ear pigs.
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