首页|大蝎子草总多酚提取工艺优化及抗氧化活性

大蝎子草总多酚提取工艺优化及抗氧化活性

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
本试验采用超声辅助乙醇提取大蝎子草地上部分总多酚，通过单因素试验结果设计Box-Behnken模型，优化大蝎子草地上部分总多酚最佳工艺条件，用DPPH自由基清除率、FRAP法评价大蝎子草地上部分总多酚的抗氧化性。结果显示，乙醇浓度、液固比、超声时间、超声温度的最佳工艺参数分别为75％、50 mL/g、35min、40 ℃时，总多酚提取率为2。81％，与Box-Behnken模型的预测值相符。抗氧化性试验表明，0。15 mg/mL大蝎子草总多酚提取液DPPH清除率可达75。8％，半数抑制浓度IC50值为0。057 mg/mL，具有一定的抗氧化性，为大蝎子草野生资源的研究和利用提供相应的理论支撑，为新型饲料添加剂的开发提供理论基础。
Optimization of Extraction Process and Antioxidant Activity of Total Polyphenols from Girardinia diversifolia
The ultrasonic-assisted ethanol was used to extract the total polyphenols from the aerial part of Girardinia diversifolia in this experiment.Box-Behnken model was designed based on the results of single-factor experiments to optimize the optimal process conditions of total polyphenols from the aerial part of Girardinia diversifolia.DPPH free radical scavenging rate and FRAP method were used to evalu-ate the antioxidant capacity of total polyphenols from the aerial part of Girardinia diversifolia.Results showed when the optimal process parameters as ethanol concentration,liquid-solid ratio,ultrasonic time and ultrasonic temperature were 75％,50 mL/g,35 min and 40 ℃,respectively,the extraction rate of to-tal polyphenols was 2.81％,which was consistent with the predicted value of Box-Behnken model.The an-tioxidant test showed that the DPPH radical-scavenging activity of 0.15 mg/mL total polyphenol from the aerial part of Girardinia diversifolia could reach 75.8％,and the IC50 value of the half inhibitory concen-tration was 0.057 mg/mL,which had certain antioxidant capacity.It provides corresponding theoretical da-ta support for further research and utilization of wild resources of Girardinia diversifolia,and a theoreti-cal basis for the development of new feed additives.

total polyphenolsGirardinia diversifoliaultrasonic extractionantioxidant

高林晓、石景海、勾娜、伍泽波、管永庆、郭蒙

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黔南民族师范学院化学化工学院,贵州都匀 558000

总多酚 大蝎子草 超声提取 抗氧化

贵州省教育厅青年科技人才成长项目贵州省教育厅青年科技人才成长项目国家级大学生创新创业训练项目黔南民族师范学院校级科研项目贵州省普通高校民族药用植物资源开发工程研究中心开放基金

黔教合KY字[2018]440[2015]3892021106700052020qnsy14黔教合KY字[2014]227

2024

10.3969/j.issn.1673-1182.2024.06.008

家畜生态学报
西北农林科技大学

家畜生态学报

CSTPCD北大核心
影响因子：0.635
ISSN：1673-1182
年,卷(期)：2024.45(6)