摘要
为了研究发酵黄芪对肉鸡小肠黏膜上皮杯状细胞数量及MUC2基因表达的影响,随机选择1日龄且初始体重相近的健康肉鸡60羽,分为4组,分别为未发酵黄芪组、发酵黄芪组、乳酸菌组和空白对照组,试验周期为42 d,于21日龄和42日龄屠宰取样.分别利用石蜡切片-PAS染色和RT-PCR方法,计数各试验组小肠黏膜上皮杯状细胞数量及其分泌的黏蛋白MUC2基因表达的差异性变化.结果显示:与空白对照组相比,发酵黄芪组21日龄肉鸡十二指肠、空肠和回肠黏膜中杯状细胞数量分别增加了 61.30％、77.63％、61.75％(P＜0.01),其分泌的黏蛋白MUC2基因的相对表达分别提高了 67.89％、64.35％、67.14％(P＜0.01);发酵黄芪组42日龄肉鸡十二指肠、空肠和回肠黏膜中杯状细胞数量分别增加了 70.89％、56.38％、40.18％(P＜0.01),其分泌的黏蛋白MUC2基因的相对表达量分别提高了 58.21％、54.61％、56.06％(P＜0.01);未发酵黄芪组也有所提高(P＜0.05),而乳酸菌组略有差异.结果表明:在肉鸡生长过程中,发酵黄芪能够提高各段小肠黏膜上皮内杯状细胞数量以及黏蛋白MUC2基因的相对表达量,且其效果优于乳酸菌和未发酵黄芪.
Abstract
In order to study the effects of fermentation astragalus on the number of goblet cells and the expression of MUC2 gene in small intestinal mucosa of broilers,60 healthy one-day-old broilers with simi-lar initial body weight were selected and randomly divided into 4 groups,namely,unfermented astragalus group,fermented astragalus group,lactobacillus group and blank control group,respectively.The experi-ment lasted for 42 days,and samples were collected at 21 and 42 days of age.The number of goblet cell was counted by paraffin section-PAS staining and the expression of MUC2 gene was detected using RT-PCR,and the differences among the experimental groups was analyzed.The results showed compared with the control group,the number of goblet cells in duodenum,jejunum and ileum mucosa of 21-day-old broil-ers in fermented astragalus group increased by 61.30％,77.63％and 61.75％respectively,and the relative expression of MUC2 gene in each segment of small intestinal mucosa of broilers increased by 67.89％,64.35％and 67.14％respectively(P＜0.01);the number of goblet cells in duodenum,jejunum and ileum mu-cosa of 42-day-old broilers in fermented astragalus group increased by 70.89％,56.38％,40.18％(P＜0.01)respectively,and the relative expression of MUC2 gene in each segment of small intestinal mucosa of broilers increased by 58.21％,54.61％,56.06％respectively(P＜0.01).And those in unfermented astrag-alus group also increased(P＜0.05),with a slight difference in lactobacillus group.The results indicated that fermented astragalus could increase the number of intraepithelial goblet cells and the relative expres-sion of MUC2 gene in all segments of small intestinal mucosa,and the effect is better than lactobacillus and unfermented astragalus.
基金项目
河南省高等学校青年骨干教师培养计划项目(2018GGJS164)
河南省科技攻关项目(222102110334)
河南省高等学校教学名师项目(教高[2020]434号)
河南省高等学校重点科研项目(22B230004)
国家科技期刊平台
NSTL
万方数据