Screening and Genetic Analysis of ASIP Gene SNP Loci in Different Tibetan Sheep Populations
Tibetan sheep are mainly distributed in the Qinghai-Tibet Plateau and its adjacent areas,with a distinctive external appearance.About 90％of the total population are mixed-colored,while pure white and pure black individuals are rare.In order to study the relationship between SNPs of ASIP gene and the heredity of Tibetan wool color,this study used pooled sequencing and PCR-SSCP methods to de-tect the polymorphisms of seven exons of the ASIP gene in four ecological types of Tibetan sheep popula-tions,including the plateau type,the Oula type,the Zhashijia type,and the black Tibetan sheep.Results showed that five mutation sites were found,including g.49396C＞G in exon 3 and g.76594G＞C,g.76715T＞A,g.76732C＞G,and g.76811C＞A in exon 7.The mutation site g.76715T＞A caused the conversion of cysteine to serine at position 126(C126S).The mutation site g.49396C＞G had three genotypes(CC,CG,and GG)in all four ecological types of Tibetan sheep,with the minimum mutation probabilities of 0.416,0.234,0.261,and 0.307,and the polymorphic information contents of 0.485,0.358,0.386,and 0.424,re-spectively,indicating moderate polymorphism.Except for the Oula type,the plateau type,black Tibetan sheep,and Zhashijia type were all in Hardy-Weinberg equilibrium.The results showed that the g.49396C＞G and g.76715T＞A missense mutations in ASIP gene are important sites regulating the inheritance of Tibetan wool color,which can serve as potential genetic markers for the breeding of Tibetan wool color.
万方数据
维普
