Genotyping and Phylogenetic Analysis of Cryptosporidium parvum from Dairy Cows in Shaanxi Province
Cryptosporidium,a zoonotic protozoon infecting host animals via the contaminated ingestion of food,water,and other sources,parasitize the digestive tracts of humans and various animals and cause watery diarrhea and even death of infected host.Cryptosporidium parvum is one of the most virulent spe-cies in the genus Cryptosporidium,and previous studies also have shown that the virulence of C.parvum have strong relevance to its genotype.Based on gp60 gene locus,this study therefore utilized molecular bi-ological methods to identify subtypes and analyze genetic evolution of C.parvum isolated from dairy cattle in Shaanxi Province.The results showed that C.parvum isolate(SX-1)from dairy cattle in this study was identified as the genotype Ⅱ dA19Gl belonging to the Ⅱ d subtype family.According to the phylogenetic relationship tree constructed,the SX-1 isolates here was grouped in one separate branch with the MH049734,MN304768,MT156342 and AY738194 isolates in GenBank as the Ⅱd subtype family.In con-clusion,these findings revealed the wide distribution and genetic diversity of C.parvum in dairy cattle in Shaanxi province,and the significance of developing accurate strategies for the prevention and control of calf diarrhea.Additionally,it provides theoretical basis and data support for the formulation of effective prevention and control strategies for C.parvum from dairy cattle in Shaanxi Province.
C.parvumgenotypingphylogenetic analysisdairy cattleShaanxi province
万方数据
维普
