In order to explore the effect of FecB gene mutation on the effect of simultaneous estrus-su-perovulation in sheep,statistical studies on estrus time,follicle number,hormones concentration and oxi-dative stress indexes in peripheral serum for three FecB genotypes of Small Tail Han sheep before and af-ter the superovulation treatment were conducted.The results showed that the estrus time of the sheep group stimulated by superovulation hormone was more concentrated than the non-superovulation treatment group;and the number of preovulatory large follicles formed in BB and+B sheep which carrying FecB gene mutation was significantly higher than that of++wild type under stimulation by superovulation hormone(P<0.05).At 6 hours after estrus,the peripheral serum FSH concentration of BB sheep treated with superovulation was significantly higher than that of other groups(P<0.05),indicating that the FSH concentration in the early estrus may determine the number of subsequent follicle formation.There was no significant difference in peripheral serum oxidative stress levels among the three FecB genotype sheep pop-ulations after superovulation treatment(P>0.05);while the higher level of serum MDA for the non-su-perovulation in BB genotype sheep reflects the higher number of follicles formed may cause a considerable degree of high oxidative stress in the sheep under normal physiological levels.