家畜生态学报2024,Vol.45Issue(11) :43-47.DOI:10.3969/j.issn.1673-1182.2024.11.008

FecB基因突变对绵羊超排处理效果的影响

Effects of FecB Gene Mutation on Superovulation Treatment in Sheep

郭晓飞 钟荣珍 张效生 张金龙 王翔宇 贺小云 储明星 房义
家畜生态学报2024,Vol.45Issue(11) :43-47.DOI:10.3969/j.issn.1673-1182.2024.11.008

FecB基因突变对绵羊超排处理效果的影响

Effects of FecB Gene Mutation on Superovulation Treatment in Sheep

郭晓飞 1钟荣珍 2张效生 3张金龙 3王翔宇 4贺小云 4储明星 4房义2
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作者信息

  • 1. 中国科学院东北地理与农业生态研究所/吉林省饲料加工与草食家畜精准饲养跨区域合作科技创新中心/吉林省草地畜牧重点实验室,吉林长春 130102;天津市农业科学院畜牧兽医研究所/天津市畜禽分子育种与生物技术重点实验室/天津市畜禽健康养殖工程技术中心,天津 300381
  • 2. 中国科学院东北地理与农业生态研究所/吉林省饲料加工与草食家畜精准饲养跨区域合作科技创新中心/吉林省草地畜牧重点实验室,吉林长春 130102
  • 3. 天津市农业科学院畜牧兽医研究所/天津市畜禽分子育种与生物技术重点实验室/天津市畜禽健康养殖工程技术中心,天津 300381
  • 4. 中国农业科学院北京畜牧兽医研究所/畜禽生物育种全国重点实验室,北京 100193
  • 折叠

摘要

为了探究FecB基因突变对绵羊同期发情-超数排卵效果的影响,基于同期发情、超数排卵等技术处理,对3种FecB基因型小尾寒羊进行了处理前后的发情时间和卵泡数量统计研究,分析其外周血清激素和氧化应激指标的差异.结果表明,相比于同期非超排试验处理,超排组绵羊群体的发情时间更为集中;且携带FecB基因突变的BB和+B型绵羊群体,在超排激素作用下所形成的排卵前卵泡数显著高于++野生型(P<0.05).在发情后6 h超排处理的BB型绵羊外周血清FSH浓度水平显著高于其他各组(P<0.05),预示着绵羊发情早期的FSH浓度可能决定着随后的卵泡形成数量.超排处理后的3种FecB基因型绵羊群体之间的外周血清氧化应激水平差异不显著(P>0.05);而非超排处理的BB型较高的血清MDA水平,反应了正常生理水平下BB型绵羊较高的卵泡形成数量可能引起了机体的相当程度的高氧化应激状态.

Abstract

In order to explore the effect of FecB gene mutation on the effect of simultaneous estrus-su-perovulation in sheep,statistical studies on estrus time,follicle number,hormones concentration and oxi-dative stress indexes in peripheral serum for three FecB genotypes of Small Tail Han sheep before and af-ter the superovulation treatment were conducted.The results showed that the estrus time of the sheep group stimulated by superovulation hormone was more concentrated than the non-superovulation treatment group;and the number of preovulatory large follicles formed in BB and+B sheep which carrying FecB gene mutation was significantly higher than that of++wild type under stimulation by superovulation hormone(P<0.05).At 6 hours after estrus,the peripheral serum FSH concentration of BB sheep treated with superovulation was significantly higher than that of other groups(P<0.05),indicating that the FSH concentration in the early estrus may determine the number of subsequent follicle formation.There was no significant difference in peripheral serum oxidative stress levels among the three FecB genotype sheep pop-ulations after superovulation treatment(P>0.05);while the higher level of serum MDA for the non-su-perovulation in BB genotype sheep reflects the higher number of follicles formed may cause a considerable degree of high oxidative stress in the sheep under normal physiological levels.

关键词

FecB基因/超数排卵/发情/生殖激素/氧化应激

Key words

FecB gene/superovulation/estrus/reproductive hormones/oxidative stress

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出版年

2024
家畜生态学报
西北农林科技大学

家畜生态学报

CSTPCD北大核心
影响因子:0.635
ISSN:1673-1182
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