The Role of Pantothenic Acid and its Downstream Fatty Acid Oxidation Metabolism in the Differentiation Process of Chicken ESCs into PGCs
This article aims to investigate the roles of differential metabolites pantothenic acid(PA)and fatty acid oxidation(FAO)in the differentiation of chicken embryonic stem cells(ESCs)into primor-dial germ cells(PGCs),and to provide a new theoretical basis for analyzing the mechanism of PGCs for-mation from a metabolic perspective.Using BMP4 induction system,five groups were established based on the optimal concentrations of pantothenic acid(PA)and its inhibitor(PZ)and fatty acid oxidation activa-tor(BMS)/inhibitor(Per)in previous studies of the research group:Control,PA+BMS,PA+Per,PZ+BMS,and PZ+Per.Cell morphology was observed,PGCs-specific marker genes were detected by qRT-PCR,and indirect immunofluorescence and flow cytometry were employed to explore the effects of panto-thenate metabolism and its downstream fatty acid oxidation during ESCs to PGCs differentiation.Cell mor-phology observations at day 6 of induction showed a significant increase in embryoid bodies in the PZ+BMS group(P＜0.01)and a significant decrease in the PA+Per group(P＜0.05).qRT-PCR results indi-cated a significant increase in CVH and C-KIT expression in the PZ+BMS-induced group(P＜0.01),while their expression was significantly reduced in the PA+Per group(P＜0.01).Indirect immunofluores-cence and flow cytometric sorting results demonstrated a significantly higher proportion of DDX4-positive cells in the PZ+BMS group compared to the control(P＜0.05),whereas the PA+Per group showed a significantly lower proportion(P＜0.01).These findings suggest that inhibiting pantothenic acid metabo-lism and activating fatty acid metabolism can promote PGCs formation.This study provides new insights into the optimization of in vitro induction culture system of PGCs using metabolites.
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维普
